Abstract: The present invention relates to the field of microbiology, specifically the field of production of a dairy food product using fermentation and the field of gene transfer by conjugation.

Abstract: The present invention relates to the field of microbiology, specifically the field of production of a dairy food product using fermentation and the field of gene transfer by conjugation.

Abstract: The present invention relates to the field of microbiology, specifically the field of production of a dairy food product using fermentation and the field of gene transfer by conjugation.

Abstract: The present invention relates to the field of microbiology, specifically the field of production of a dairy food product using fermentation and the field of gene transfer by conjugation.

Abstract: According to an example aspect of the present invention, there is provided an apparatus comprising a random access memory device, at least one processing core coupled via a first interface with the random access memory device, and a secure hardware element, comprising hash function circuitry, and coupled directly via a second interface with the random access memory device, the secure hardware element configured to obtain as input data from a memory space of the random access memory device, to produce as output a hash value of the input, and to cryptographically sign the hash value using a physically unclonable function value of the apparatus.

Abstract: The present invention relates to the field of microbiology, specifically the field of production of a dairy food product using fermentation and the field of gene transfer by conjugation.

Abstract: Various embodiments relate to a method and apparatus for using blockchains as an integrity tracking tool for network elements, the method including the steps of receiving, by a blockchain network, a patch request from a patch initiator, validating, by the blockchain network, the patch request and writing the patch request on the blockchain, transmitting, by the blockchain network, the patch request to a network element, receiving, by the blockchain network, a patch report from the network element, and validating, by the blockchain network, the patch report and writing the patch report on the blockchain.

Abstract: Various embodiments relate to a method and apparatus for using blockchains as an integrity tracking tool for network elements, the method including the steps of receiving, by a blockchain network, a patch request from a patch initiator, validating, by the blockchain network, the patch request and writing the patch request on the blockchain, transmitting, by the blockchain network, the patch request to a network element, receiving, by the blockchain network, a patch report from the network element, and validating, by the blockchain network, the patch report and writing the patch report on the blockchain.

Abstract: Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.

Abstract: Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.

Abstract: The invention relates to surface display of proteins on microorganisms via the targeting and anchoring of heterologous proteins to the outer surface of cells such as yeast, fungi, mammalian, plant cells, and bacteria. The invention provides a proteinaceous substance comprising a reactive group and at least one attaching peptide including a stretch of amino acids having a sequence corresponding to at least a part of the consensus amino acid sequence listed in FIG. 10 and further includes a method for attaching a proteinaceous substance to the cell wall of a microorganism comprising the use of the attaching peptide.

Abstract: The invention relates to methods and means for regulating gene expression and to CodY target sequences. CodY represses its target genes by binding to specific DNA sequences upstream of the respective genes. A conserved target site was identified by analyzing upstream sequences of derepressed genes in a delta codY L. lactis MG1363 derivative, as identified in a DNA micro-array study. Also, CodY target sequences from other gram-positive bacteria, like B. subtilis and Streptococcus are disclosed. Hence, the invention provides CodY target sequences that may be used in different applications to repress or derepress gene expression.

Abstract: The invention relates to surface display of proteins on microorganisms via the targeting and anchoring of heterologous proteins to the outer surface of cells such as yeast, fungi, mammalian, plant cells, and bacteria. The invention provides a proteinaceous substance comprising a reactive group and at least one attaching peptide including a stretch of amino acids having a sequence corresponding to at least a part of the consensus amino acid sequence listed in FIG. 10 and further includes a method for attaching a proteinaceous substance to the cell wall of a microorganism comprising the use of the attaching peptide.

Abstract: The invention relates to surface display of proteins on micro-organisms via the targeting and anchoring of heterologous proteins to the outer surface of cells such as yeast, fungi, mammalian and plant cells, and bacteria. The invention provides a proteinaceous substance comprising a reactive group and at least one attaching peptide which comprises a stretch of amino acids having a sequence corresponding to at least a part of the consensus amino acid sequence listed in FIG. 10 and comprises a method for attaching a proteinaceous substance to the cell wall of a micro-organism comprising the use of said attaching peptide.

Abstract: Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.

Abstract: Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.

Abstract: The invention relates to surface display of proteins on microorganisms via the targeting and anchoring of heterologous proteins to the outer surface of cells such as yeast, fungi, mammalian, plant cells, and bacteria. The invention provides a proteinaceous substance comprising a reactive group and at least one attaching peptide including a stretch of amino acids having a sequence corresponding to at least a part of the consensus amino acid sequence listed in FIG. 10 and further includes a method for attaching a proteinaceous substance to the cell wall of a microorganism comprising the use of the attaching peptide.

Abstract: A computer memory for storing an n-dimensional object for access by an application program being executed on a data processing system comprising a data structure stored in the memory including information about the n-dimensional object used by the application program and including a first pitch selector node including a plurality of first pitch selector branches, each of the first pitch selector branches representing a pitch.

Abstract: A method of identifying objects intersecting a search window comprises (i) searching each structure associated with each element of a pitch selector; (ii) for each of the structures, searching all subspaces that intersect the search window; and (iii) within each of the subspaces, identifying objects intersecting the search window.

Abstract: A method of storing an n-dimensional object in a data structure includes defining a scale, the scale comprising a plurality of pitches, each of the pitches defined by a boundary spacing distance and initial offset, partitioning a space by each of the pitches into a plurality of subspaces, at least one of the pitches partitioning the space along a particular dimension into three or more of the subspaces, and storing the object into one of the subspaces.