Abstract: The present application relates to methods of treating a laminopathy affecting skeletal or cardiac muscle in a subject. The methods involve selecting a subject who has a laminopathy affecting skeletal or cardiac muscle. In some embodiments, the methods involve administering, to the selected subject, an inhibitor of a protein associated with a DNA damage response (DDR) pathway to treat the laminopathy affecting skeletal or cardiac muscle in the subject. In other embodiments, the methods involve administering, to the selected subject, a microtubule stabilizing agent and a Linker of Nucleoskeleton and Cytoskeleton (LINC) complex disruptor to treat the laminopathy affecting skeletal or cardiac muscle in the subject. Also disclosed are pharmaceutical compositions comprising an inhibitor of a protein associated with a DNA damage response (DDR) pathway, a microtubule stabilizing agent, and/or a Linker of Nucleoskeleton and Cytoskeleton (LINC) complex disruptor.

Abstract: An active three-dimensional imaging system for optical apparatus includes a relay lens subsystem with an off-axis rotating aperture placed between an imaging or objective lens and an image recording device such as a CCD camera. According to another aspect, the imaging system can include an off-axis rotating aperture located between field and aperture diaphragms of an illumination subsystem. The rotating aperture allows adjustable non-equilateral spacing between images of out-of-focus object points to achieve higher spatial resolution, increased sensitivity and higher sub-pixel displacement accuracy than other systems.