Abstract: A 3D tissue culture selected from the group consisting of hydrogel-based 3D tissue culture and cellular self-assembly 3D tissue culture as well as self-assembly 3D tissue culture. Additionally, disclosed is a method of preparing cells for 3D tissue culture, which method comprises the steps of plating the cells on a suitable surface, optionally, checking for their capability to adhere to said surface, discarding the cells which have not adhered to said surface, detaching the adhered cells and transferring them into a 3D tissue culture process.

Abstract: Disclosed herein is a transport device comprising a first section of an outer container comprising a shell on the inside having the shape of a spherical cap with an inner diameter and an opening, wherein the opening of the spherical cap faces upwards, and an inner container having an upper section, a lower section and an inner hollow volume defined thereby, wherein at least the lower section has a spherical shape on the outside, the outer diameter of which is smaller than the inner diameter of the spherical cap of the outer container. The inner container is suitable to be arranged in the spherical cap of the outer container in a freely pivotable fashion, and is capable of accommodating a payload.

Abstract: Provided is a system and a method for propagating cells, the system comprising a multi well plate and a plate sealing means for sealing at least one well of a multiwell plate, the plate sealing means comprising at least one solid bulge or at least one bulge comprising a solid base, said at least one bulge consisting of a resilient elastomer to securely seal at least one well of the multiwell plate. Also disclosed is the use of such a system for propagating cells and the corresponding method.

Abstract: Disclosed herein is a transport device comprising a first section of an outer container comprising a shell on the inside having the shape of a spherical cap with an inner diameter and an opening, wherein the opening of the spherical cap faces upwards, and an inner container having an upper section, a lower section and an inner hollow volume defined thereby, wherein at least the lower section has a spherical shape on the outside, the outer diameter of which is smaller than the inner diameter of the spherical cap of the outer container. The inner container is suitable to be arranged in the spherical cap of the outer container in a freely pivotable fashion, and is capable of accommodating a payload.

Abstract: Provided is a system and a method for propagating cells, the system comprising a multi well plate and a plate sealing means for sealing at least one well of a multiwell plate, the plate sealing means comprising at least one solid bulge or at least one bulge comprising a solid base, said at least one bulge consisting of a resilient elastomer to securely seal at least one well of the multiwell plate. Also disclosed is the use of such a system for propagating cells and the corresponding method.

Abstract: Disclosed are devices for propagating at least one microtissue, methods for manufacturing such devices, and the use us such devices, said devices comprise at least one well, wherein said at least one well comprises an open upper section comprising an open upper end and an open lower end, and a lower section comprising an open upper end and a bottom end, wherein the upper section and the lower section are in fluid communication, and wherein the bottom end of the lower section of at least one well of said plurality of wells is provided with a well bottom, said lower section of said at least one well has a smaller cross-sectional area than the open upper section of the same well.

Abstract: The present invention relates to an in vitro tumor relapse assay method, which method comprises providing a 3-dimensional cell culture or tissue comprising immortalized, malignant, cancerous and/or neoplastic cells, exposing the 3-dimensional cell culture or tissue to at least one anti-cancer agent, interrupting the exposure and determining the effect of said exposure and interruption on the 3-dimensional cell culture or tissue.

Abstract: Disclosed are devices for propagating at least one microtissue, methods for manufacturing such devices, and the use us such devices, said devices comprise at least one well, wherein said at least one well comprises an open upper section comprising an open upper end and an open lower end, and a lower section comprising an open upper end and a bottom end, wherein the upper section and the lower section are in fluid communication, and wherein the bottom end of the lower section of at least one well of said plurality of wells is provided with a well bottom, said lower section of said at least one well has a smaller cross-sectional area than the open upper section of the same well.

Abstract: Provided is a system and a method for propagating cells, the system comprising a multi well plate and a plate sealing means for sealing at least one well of a multiwell plate, the plate sealing means comprising at least one solid bulge or at least one bulge comprising a solid base, said at least one bulge consisting of a resilient elastomer to securely seal at least one well of the multiwell plate. Also disclosed is the use of such a system for propagating cells and the corresponding method.

Abstract: The present invention relates to a method of preparing cells for 3D tissue culture, which method comprises the steps of plating the cells on a suitable surface, optionally, checking for their capability to adhere to said surface, discarding the cells which have not adhered to said surface, detaching the adhered cells and transferring them into a 3D tissue culture process.

Abstract: Multi-well plate (1) comprising a plurality of wells (4), a frame (5) for holding the plurality of wells (4) in a defined arrangement, a support element (50) for contacting a surface when the multi-well plate (1) is placed on the surface, wherein at least one well (4) of the plurality of wells is elastically displaceable relative to said support element (50).

Abstract: Multi-well plate (1) comprising a plurality of wells (4), a frame (5) for holding the plurality of wells (4) in a defined arrangement, a support element (50) for contacting a surface when the multi-well plate (1) is placed on the surface, wherein at least one well (4) of the plurality of wells is elastically displaceable relative to said support element (50).

Abstract: A (bio-)chemical assay on sensing objects (4) e.g. for use as a drug screening assay on living cells, as well uses and a method for making such an integrated system is proposed.

Abstract: An integrated lab-on-a-chip diagnostic system for carrying out a sample preparation process on a fluid sample containing cells and/or particles, the system comprising: (a) an inlet for a fluid sample; (b) a lysis unit for lysis of cells and/or particles contained in the fluid sample; (c) a nucleic acid extraction unit for extraction of nucleic acids from the cells and/or particles contained in the fluid sample; (d) a reservoir containing a lysis fluid; (e) a reservoir containing an eluent for removing nucleic acids collected in the nucleic acid extraction unit; wherein the sample inlet is in fluid communication with the lysis unit, an optional valve being present to control the flow of fluid therebetween; wherein the lysis unit is in fluid communication with the nucleic acid extraction unit, an optional valve being present to control the flow of fluid therebetween; wherein the reservoir containing the lysis fluid is in fluid communication with the lysis unit, an optional valve being present to control the flo

Abstract: A diving computer is provided. The diving computer includes a computer connected to a dot matrix screen to display information concerning a dive. The computer has an interface to an external personal computer for reprogrammably configuring the information displayed on the screen. The user may customize the information on one or more views of the screen in several ways. The user may select the desired information to be displayed, and may customize the size and placement of the information on one or more views. The diving computer also has a number of pre-programmed displays that may be selected, each of which present different layouts and organizations of diving related information.

Abstract: A dive computer that includes heart rate monitoring capability is provided. The heart rate monitoring is accomplished by a belt that fits around the diver's chest, and the information is wirelessly transmitted to the dive computer. The heart rate information may be displayed during and after a dive, and the dive computer may selectively utilize the monitored heart rate information to compensate the decompression algorithm based on workload during the dive. The dive computer can also utilize the monitored heart rate information to compensate the decompression algorithm based on workload on the surface before and/or after the dive for no fly and repetitive dive calculations.

Abstract: A diving computer is provided. The diving computer includes a computer connected to a dot matrix screen to display information concerning a dive. The computer has an interface to an external personal computer for reprogrammably configuring the information displayed on the screen. The user may customize the information on one or more views of the screen in several ways. The user may select the desired information to be displayed, and may customize the size and placement of the information on one or more views. The diving computer also has a number of pre-programmed displays that may be selected, each of which present different layouts and organizations of diving related information.

Abstract: A microfabricated device for fragmenting nucleic acids present in a fluid sample, the device comprising an inlet port (20), a fragmentation cell (1), and an outlet port (10) downstream from said inlet port (20), said cell (1) being in fluid communication with said ports, and wherein said outlet port is dimensioned to impede the flow of a fluid sample out of said cell (1) so as to effect shearing of nucleic acids molecules therein.