Patents by Inventor Jan Trnovsky
Jan Trnovsky has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20160257997Abstract: This application pertains to methods for the whole-cell analysis of gram-positive bacteria. The methods are capable of making a determination of whether or not a sample (e.g., a clinical sample) comprises one or more select gram-positive bacteria as well as, for example, whether or not none, some or all of said select gram-positive bacteria in said sample, or possibly other bacteria in said sample, possess a select trait or traits of interest. In some embodiments, the methods can be used to determine methicillin-resistant (the select trait) staphylococcus aureus (the select gram-positive bacteria), coagulase-negative staphylococci (another select gram-positive bacteria) and/or methicillin-sensitive staphylococcus aureus (MSSA) in said sample. The whole-cell analysis can be performed, for example, by in-situ hybridization (ISH), fluorescence in-situ hybridization (FISH), immunocytochemistry (ICC), or any combination of two or more of the foregoing.Type: ApplicationFiled: October 13, 2015Publication date: September 8, 2016Applicant: AdvanDx, Inc.Inventors: Henrik Stender, Jan Trnovsky, Lisa L. Klimas, Anne K.I. Rasmussen
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Publication number: 20160060688Abstract: This invention pertains to identifying one or more hybridization probes sequestered within (or optionally released from the intact) cells or microorganisms by mass spectrometry to thereby determine a trait of the cells or microorganisms and/or to identify the cells or microorganisms themselves. The cells or microorganisms can come from a subject and the information obtained from the mass spectrometry analysis may, if clinically relevant, optionally be used to diagnose and/or treat the subject.Type: ApplicationFiled: June 10, 2015Publication date: March 3, 2016Applicant: ADVANDX, INC.Inventors: James M. Coull, Martin Fuchs, Mark J. Fiandaca, Alisha Perelta, Jan Trnovsky
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Publication number: 20120052499Abstract: This application pertains to methods for the whole-cell analysis of gram-positive bacteria. The methods are capable of making a determination of whether or not a sample (e.g. a clinical sample) comprises one or more select gram-positive bacteria as well as, for example, whether or not none, some or all of said select gram-positive bacteria in said sample, or possibly other bacteria in said sample, possess a select trait or traits of interest. In some embodiments, the methods can be used to determine methicillin-resistant (the select trait) staphylococcus aureus (the select gram-positive bacteria), coagulase-negative staphylococci (another select gram-positive bacteria) and/or methicillin-sensitive staphylococcus aureus (MSSA) in said sample. The whole-cell analysis can be performed, for example, by in-situ hybridization (ISH), fluorescence in-situ hybridization (FISH), immunocytochemistry (ICC), or any combination of two or more of the foregoing.Type: ApplicationFiled: May 20, 2010Publication date: March 1, 2012Applicant: AdvanDx, Inc.Inventors: Henrik Stender, Jan Trnovsky, Lisa L. Klimas, Anne K.I. Rasmussen
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Patent number: 7556928Abstract: The invention provides methods of analyzing a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.Type: GrantFiled: August 13, 2004Date of Patent: July 7, 2009Assignee: One Cell Systems, Inc.Inventors: Diana Jespersen, Yevgenya Akselband, Patricia McGrath, Jan Trnovsky, Phillip T. Moen
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Publication number: 20080171329Abstract: The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.Type: ApplicationFiled: October 26, 2007Publication date: July 17, 2008Applicant: Cellay, LLC c/o One Cell Systems, Inc.Inventors: Jan Trnovsky, Patricia McGrath
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Publication number: 20080160498Abstract: The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.Type: ApplicationFiled: October 5, 2007Publication date: July 3, 2008Applicant: Cellay, LLC c/o One Cell Systems, Inc.Inventors: Jan Trnovsky, Patricia McGrath
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Publication number: 20070020617Abstract: The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.Type: ApplicationFiled: April 4, 2006Publication date: January 25, 2007Applicant: Cellay, LLC c/o One Cell Systems, Inc.Inventors: Jan Trnovsky, Patricia McGrath
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Publication number: 20050059049Abstract: The present invention provides nucleic acid hybridization probes having a target-binding region and a labeled hairpin structure at at least one end of the probe. The hairpin-labeled probes include oligonucleotides, dendrimers, and primer-extended nucleic acids. The probes can be used in disclosed methods for detection of target nucleic acids. In addition, the oligonucleotide probes can be used in disclosed methods for primer-extension, including, e.g., random priming and PCR amplification, to produce the primer-extended hairpin-labeled probes. Also disclosed are kits comprising the hairpin-labeled oligonucleotide and dendrimer probes. Further, the present invention provides biomolecules (e.g., peptides, polypeptides, carbohydrates, lipids, and the like) that are labeled via linkage to labeled hairpin structures.Type: ApplicationFiled: July 7, 2004Publication date: March 17, 2005Applicant: One Cell Systems, Inc.Inventors: Phillip Moen, Jan Trnovsky
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Publication number: 20050019839Abstract: The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.Type: ApplicationFiled: August 13, 2004Publication date: January 27, 2005Applicant: Once Cell Systems, Inc.Inventors: Diana Jespersen, Yevgenya Akselband, Patricia McGrath, Jan Trnovsky, Phillip Moen
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Patent number: 6806058Abstract: The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.Type: GrantFiled: May 28, 2002Date of Patent: October 19, 2004Assignee: One Cell Systems, Inc.Inventors: Diana Jesperson, Yevgenya Akselband, Patricia McGrath, Jan Trnovsky, Phillip T. Moen
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Publication number: 20030207260Abstract: The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.Type: ApplicationFiled: May 2, 2003Publication date: November 6, 2003Applicant: Cellay, LLC c/o One Cell Systems, Inc.Inventors: Jan Trnovsky, Patricia McGrath
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Publication number: 20030143642Abstract: The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.Type: ApplicationFiled: May 28, 2002Publication date: July 31, 2003Applicant: One Cell Systems, Inc.Inventors: Diana Jesperson, Yevgenya Akselband, Patricia McGrath, Jan Trnovsky, Phillip T. Moen
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Patent number: 6586176Abstract: The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.Type: GrantFiled: August 6, 1999Date of Patent: July 1, 2003Assignee: Cellay, LLCInventors: Jan Trnovsky, Patricia McGrath