Abstract: Methods are provided for determining epimutations in a nucleic acid sequence of a cell.

Abstract: Methods are provided for determining if an agent causes somatic mutations in a genome, and kits, systems and computer-readable medium therefor.

Abstract: Primer sequences and materials are pre-prepared as test kits for enabling appropriate gene scanning patterns, preferably by two dimensional electrophoresis (TDGS), for use in detecting sequence variations and/or mutations in BRCA1 and h MLH1 genes

Abstract: The invention encompasses a semi-automatic and/or automatic computer-aided technique for designing comprehensive DNA diagnostic tests for mutations in disease genes through searching for optimized conditions for PCR amplification and for optimal melting behavior in denaturing gradient gels and for optimal distribution in two-dimensional electrophoresis display of the mutational target fragments.

Abstract: A novel method and test kit for mutagenesis testing applicable to an organism or a cell.

Abstract: An assay for detecting mutations in genes by a two-step multiplex polymerase chain reaction amplification followed by preferably two-dimensional electrophoretic separation of the fragments on the basis of both size and base pair sequence.

Abstract: Detection of mutations in a lacZ gene including lacZ operator sequence functioning as marker gene located in a plasmid whereof one or more copies are introduced into the DNA of a mammal or of a mammalian cell, by isolating the DNA, cutting the plasmid out of the DNA, picking up the plasmid DNA with solid particles to which a lacZ operator binding material is bound, again releasing the plasmid from the solid particles after isolation and subsequently circularizing the plasmid, transforming a host restriction-negative, lacZ-negative and galE-negative bacterial host with the plasmid and separating transformants with mutated lacZ gene which grow on a lactose-containing medium from transformants with a non-mutated lacZ gene which do not grow on such a medium. A transgenic mammal model for mutation analysis.

Abstract: The invention relates to an apparatus for two-dimensional electrophoresis of at least one electrophoresis unit comprising an electrophoresis medium enclosed between two plates, which apparatus comprises a first pair and a second pair of compartments for electrophoresis liquid which are provided with electrodes and which make electrophoretic contact on either side and mutually transversely of each other with the electrophoresis medium of the electrophoresis unit, wherein the compartments are disposed and adapted such that the electrophoresis unit assumes a standing position in the apparatus, and to an electrophoresis unit intended for such an apparatus.

Abstract: The invention relates to a process for the rescue and cloning of a DNA sequence wherein a vector containing said DNA sequence is multiplied in a suitable bacterial host. As the bacterial host, a bacterial strain which is incapable of host restriction is used, such as an Escherichia coli C strain. Preferably, a bacteriophage vector which is a suitable cloning vehicle for the bacterial host concerned is used as the vector, the step of in vitro packaging into phage coats being performed by means of a phage packaging extract obtained from a bacterial strain which is incapable of host restriction.

Abstract: This invention relates to a method of simultaneously determining DNA sequence variations on a large number of loci, and a kit suitable therefor. The method comprises an electrophoretic separation of restriction fragments in two dimensions on the basis of two independent criterions, namely, length and base pair sequence, and a detection of separated fragments by means of a labelled probe comprising one or more units of minisatellite sequences. Preferred probes are GC-rich minisatellite core sequences.