Abstract: The present invention relates to a method for increasing polyhydroxyalkanoates (PHAs) content of waste sludge, by taking the fermentation liquid fermented from waste sludge as a carbon source, and performing ADF domestication process, thereby quickly raising PHAs contentin the waste sludge, in accordance with the method of the present invention, the conventional complicated domestication steps is simplified and a higher content PHAs can be produced, additionally the added carbon source of the present invention is the VFA fermented by the waste sludge, so it's unnecessary to add extra material, therefore including more industrial utilization.

Abstract: The present invention provides a method for extracting polyhydroxyalkanoates (PHAs), which comprises a pre-process step and an extraction step: removing water from waste sludge containing microorganisms in the pre-process step so that the waste sludge containing microorganisms has a water content of less than 40%; and applying a high-voltage pulsed electric field to the waste sludge during the extraction step to destroy the microorganisms and release the PHAs, wherein the high-voltage pulsed electric field is between 50 volts and 400 volts, an application time of the high-voltage pulsed electric field is between 5 seconds and 90 seconds, and an application frequency of the high-voltage pulsed electric field is between 500 Hz and 1000 Hz, thereby extracting the PHAs in the case of few chemicals.

Abstract: The present invention relates to a method for increasing polyhydroxyalkanoates (PHAs) content of waste sludge, by taking the fermentation liquid fermented from waste sludge as a carbon source, and performing ADF domestication process, thereby quickly raising PHAs contentin the waste sludge, in accordance with the method of the present invention, the conventional complicated domestication steps is simplified and a higher content PHAs can be produced, additionally the added carbon source of the present invention is the VFA fermented by the waste sludge, so it's unnecessary to add extra material, therefore including more industrial utilization.

Abstract: The present invention provides a method for extracting polyhydroxyalkanoates (PHAs), which comprises a pre-process step and an extraction step: removing water from waste sludge containing microorganisms in the pre-process step so that the waste sludge containing microorganisms has a water content of less than 40%; and applying a high-voltage pulsed electric field to the waste sludge during the extraction step to destroy the microorganisms and release the PHAs, wherein the high-voltage pulsed electric field is between 50 volts and 400 volts, an application time of the high-voltage pulsed electric field is between 5 seconds and 90 seconds, and an application frequency of the high-voltage pulsed electric field is between 500 Hz and 1000 Hz, thereby extracting the PHAs in the case of few chemicals.