Abstract: The application provides devices, kits and methods for a streamlined lateral flow assay for sample preparation, optional amplification, and detection of a target nucleic acid using programmable nuclease reagents.

Abstract: A photosensitizer formulation can be disposed on or in a mesh; net; netting; screen; curtain of strands, fibers, or monofilaments; substrate, personal protective gear, mask, or any other suitable object. The photosensitizer formulation, when in contact with molecular oxygen and activated by light or ultrasound, produces microbicidal singlet oxygen. A variety of different arrangements and applications are described. For example, an air flow device may also be included to generate a flow of air through or over the photosensitizer formulation. A fluorescent formulation may be included to monitor photobleaching. The photosensitizer formulation may be disposed in a concentration gradient to generate antigenic particles by damaging or destroying microbes.

Abstract: A device for sorting cells includes a rotatable substrate (601) configured to rotate around a rotation axis (611) to generate a centrifugal force and a cover layer (441) attached to the rotatable substrate (601). The rotatable substrate (601) includes a sample reservoir (602) disposed at the center of the rotatable substrate (601), a flow path (604) coupled to the sample reservoir (602) and extending radially towards a periphery of the rotatable substrate (601) and including a bifurcation (606) into a main channel (607) and a side channel (608). The rotatable substrate (601) moves a plurality of cells stored in the sample reservoir (602) to a periphery of the rotatable substrate (601) by a centrifugal force. The cover layer (441) includes a first opening in fluid communication with the main channel (607) and a second opening in fluid communication with the side channel (608).

Abstract: A method for preparing droplets using a microfluidic chip system is provided. The microfluidic chip system includes a droplet generation device, a power generation device, a collection bottle, a connection device, and a preparation platform, the droplet generation device includes a chip body, the chip body defines a continuous phase inlet for receiving a continuous phase and a dispersed phase inlet for receiving a dispersed phase. The power generation device is activated to form a pressure difference among the collection bottle, the connection device, and the chip body, wherein the pressure difference promotes the dispersed phase and the continuous phase to converge and flow into the collection bottle in form of the droplets.

Abstract: Methods for product authenticity verification and corresponding systems and computer-readable mediums. A method includes receiving and storing product data corresponding to a product. The method includes generating and storing a validation seed associated with the product data. The method includes generating and storing a validation identifier (ID) using at least the validation seed. The method includes returning the generated validation ID. The validation ID can thereafter be used to create a validation marker that is applied to the product.

Abstract: Methods for product ownership recordation and validation and corresponding systems and computer-readable mediums. A method includes receiving product data and an owner identification (ID) corresponding to a product and generating a validation seed associated with the product data. The method includes generating a title ID using the validation seed and generating a digital title corresponding to the title ID. The digital title or title ID is stored by the data processing system as associated with the validation seed and the owner ID. The method includes returning the digital title.

Abstract: A microfluidic chip system for generating droplets is provided by the present disclosure. The microfluidic chip system includes a droplet generation device for generating the droplets, a power generation device for supplying power to the droplet generation device, a collection container for collecting the droplets flowing out of the droplet generation device, a connection device connecting the droplet generation device, the power generation device, and the collection container to each other, and a preparation platform holds together the droplet generation device, the power generation device, and the collection container. A method for preparing the droplets is also provided by the present disclosure.

Abstract: A smart and scalable dementia assistant device is provided that converses with a patient in voices familiar with the patient. It utilizes content learned from the patient and content provided by family, friends, caregivers, and doctors, and autonomously adjusts conversations based on the changing state of the patient's dementia state. The device autonomously controls IoT devices (e.g. doors, elevators, tvs, medical dispensers) to help and assist the dementia patient using oral and IoT sensors.

Abstract: A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.

Abstract: A smart and scalable dementia assistant device is provided that converses with a patient in voices familiar with the patient. It utilizes content learned from the patient and content provided by family, friends, caregivers, and doctors, and autonomously adjusts conversations based on the changing state of the patient's dementia state. The device autonomously controls IoT devices (e.g. doors, elevators, tvs, medical dispensers) to help and assist the dementia patient using oral and IoT sensors.

Abstract: A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.

Abstract: The invention provides microfluidic devices, systems, and methods for manipulating an object within a channel of a microfluidic device using an external electrode. The device has a channel disposed within the device, the channel having no included electrodes. The channel has a wall, at least a portion of which is penetrable by an electric field generated external to the device, the wall being penetrable such that the electric field extends through the wall portion and into a region within the channel. The system includes the microfluidic device and an electrode external to and not bonded to the device. In the method, the external electrode is placed adjacent to the device and energized to generate an electric field that extends through the wall of the device and into the channel, thereby manipulating an object within the channel.

Abstract: A system may generate and update a key performance indicator (KPI) on a KPI dash. The system may receive a request and access KPI data to generate a self-contained KPI object. The system can also use metadata associated with the KPI to generate a set of metadata objects to be included in the KPI object. The self-contained KPI object can be displayed on the dashboard, and responsive to a rating received from a user, a cumulative rating may be updated. The new rating may be displayed within the KPI on the dashboard.

Abstract: A system may generate and update a key performance indicator (KPI) on a KPI dash. The system may receive a request and access KPI data to generate a self-contained KPI object. The system can also use metadata associated with the KPI to generate a set of metadata objects to be included in the KPI object. The self-contained KPI object can be displayed on the dashboard, and responsive to a rating received from a user, a cumulative rating may be updated. The new rating may be displayed within the KPI on the dashboard.

Abstract: A system may generate and update a key performance indicator (KPI) on a KPI dash. The system may receive a request and access KPI data to generate a self-contained KPI object. The system can also use metadata associated with the KPI to generate a set of metadata objects to be included in the KPI object. The self-contained KPI object can be displayed on the dashboard, and responsive to a rating received from a user, a cumulative rating may be updated. The new rating may be displayed within the KPI on the dashboard.

Abstract: A system may generate and update a key performance indicator (KPI) on a KPI dash. The system may receive a request and access KPI data to generate a self-contained KPI object. The system can also use metadata associated with the KPI to generate a set of metadata objects to be included in the KPI object. The self-contained KPI object can be displayed on the dashboard, and responsive to a rating received from a user, a cumulative rating may be updated. The new rating may be displayed within the KPI on the dashboard.

Abstract: A system may generate and update a key performance indicator (KPI) on a KPI dash. The system may receive a request and access KPI data to generate a self-contained KPI object. The system can also use metadata associated with the KPI to generate a set of metadata objects to be included in the KPI object. The self-contained KPI object can be displayed on the dashboard, and responsive to a rating received from a user, a cumulative rating may be updated. The new rating may be displayed within the KPI on the dashboard.

Abstract: A system may generate and update a key performance indicator (KPI) on a KPI dash. The system may receive a request and access KPI data to generate a self-contained KPI object. The system can also use metadata associated with the KPI to generate a set of metadata objects to be included in the KPI object. The self-contained KPI object can be displayed on the dashboard, and responsive to a rating received from a user, a cumulative rating may be updated. The new rating may be displayed within the KPI on the dashboard.

Abstract: Methods and systems for reducing the run-to-run variability in measurement results obtained by a sample analysis system. The method and system utilize data from previous sample runs to optimize analysis parameters for future test runs. The methods and systems are particularly suitable for microfluidic sample analysis.

Abstract: A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.