Abstract: A sample distribution device for distribution of samples in liquid chromatographs comprises a sample supply channel arranged in a holding element. The holding element includes a receiving chamber, with the sample supply channel leading into the receiving chamber. Internally of receiving chamber, a distributing element is arranged. The passage openings for passage of the sample are formed by an inner side of the receiving chamber and by an outer side of the distributing element; preferably, for this purpose, the distributing element has recesses on the outer side. The sample distribution device is arranged in a device for liquid chromatography, wherein the sample will flow radially through the chromatography resin.

Abstract: The invention relates to a process for isolating and purifying a polynucleotide on a manufacturing scale which uses a chromatographic separation process comprising a combination of two different chromatographic steps selected from hydrophobic interaction chromatography, polar interaction chromatography and anion exchange chromatography. In at least one of the two steps the chromatographic support is a porous monolithic bed. The invention also relates to a chromatographic device and its use for isolating and purifying a polynucleotide of interest, in particular plasmid DNA, on manufacturing scale.