Patents by Inventor Janice M. Cline

Janice M. Cline has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Multi-site mutagenesis
    Patent number: 8685676
    Abstract: The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired mutant by screening large number of transformants in a short time. Moreover, the inclusion of FEN-1, PEF and optimized buffer and cycling conditions provided in the present invention should also facilitate random mutagenized library construction and the mutagenesis of large or difficult templates.
    Type: Grant
    Filed: October 11, 2011
    Date of Patent: April 1, 2014
    Assignee: Agilent Technologies, Inc.
    Inventors: Holly H. Hogrefe, Janice M. Cline
  • Multi-site mutagenesis
    Patent number: 8067556
    Abstract: The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired mutant by screening large number of transformants in a short time. Moreover, the inclusion of FEN-1, PEF and optimized buffer and cycling conditions provided in the present invention should also facilitate random mutagenized library construction and the mutagenesis of large or difficult templates.
    Type: Grant
    Filed: July 18, 2002
    Date of Patent: November 29, 2011
    Assignee: Agilent Technologies, Inc.
    Inventors: Holly H. Hogrefe, Janice M. Cline
  • Compositions and methods for random nucleic acid mutagenesis
    Patent number: 6803216
    Abstract: The invention relates to compositions and methods for nucleic acid PCR mutagenesis using novel error-prone DNA polymerases and a PCR enhancing factor. The invention also relates to compositions and methods for nucleic mutagenesis with two or more DNA polymerases lacking or exhibiting reduced exonuclease activity. The invention further relates to kit format of said compositions for PCR mutagenesis.
    Type: Grant
    Filed: May 23, 2002
    Date of Patent: October 12, 2004
    Assignee: Stratagene California
    Inventors: Holly H. Hogrefe, Janice M. Cline
  • Compositions and methods for random nucleic acid molecules
    Publication number: 20030152944
    Abstract: The invention relates to compositions and methods for nucleic acid PCR mutagenesis using novel error-prone DNA polymerases and a PCR enhancing factor. The invention also relates to compositions and methods for nucleic mutagenesis with two or more DNA polymerases lacking or exhibiting reduced exonuclease activity. The invention further relates to kit format of said compositions for PCR mutagenesis.
    Type: Application
    Filed: May 23, 2002
    Publication date: August 14, 2003
    Applicant: Stratagene
    Inventors: Holly H. Hogrefe, Janice M. Cline