Abstract: The present disclosure provides methods for treating wounds by applying a meshed autograft and skin substitute overlay wherein the skin substitute is an organotypic human skin equivalent comprising NIKS® cells.

Abstract: The present disclosure provides methods for treating acute wounds and improving outcomes by applying to an acute wound a skin substitute that is an organotypic human skin equivalent comprising NIKS cells. In certain embodiments, the closed wound has improved vascularity, improved pigmentation, decreased thickness, decreased pain, increased pliability, increased surface area, decreased stiffness, decreased itching, improved color, or any combination thereof, as assessed by an observer or by the subject, as compared to an autograft or another skin substitute.

Abstract: The present invention provides a method of repairing a tympanic membrane deformity, such as a tympanic membrane perforation, commonly referred to as tympanoplasty or myringoplasty, using a collagen biofabric. The collagen biofabric is preferably laminated. The invention further provides kits comprising one or more pieces of collagen biofabric, for example laminated collagen biofabric, for the repair of a tympanic membrane.

Abstract: The present invention provides a method of treating a leg ulcer comprising contacting the leg ulcer with a collagen biofabric. The leg ulcer may be a venous, arterial, diabetic or decubitus (pressure) ulcer. The invention further provides kits comprising one or more pieces of collagen biofabric for the treatment of a leg ulcer.