Abstract: A diffractometric sensor (1), comprises:—a substrate (3);—two interdigitated affinity gratings (2), a first affinity grating (20) comprising first unit cells (200) with affinity elements (201) and a second affinity grating (21) comprising second unit cells (210) with affinity elements (211), wherein the first unit cells (200) and second unit cells (210) are configured and arranged such that coherent light of a predetermined wavelength generated at a predetermined beam generation location (40) and diffracted by target molecules (204, 214)) bound to the affinity elements (201, 211) constructively interferes at a predetermined detection location (50) with an inverse phase, and wherein the first and second affinity gratings (20, 21) are balanced to generate a bias signal at the predetermined detection location (50) that corresponds to a difference (Am) in the scattering mass of the first and second affinity gratings (20, 21) which is in the range of 0.001 pg/mm2 to 30000 pg/mm2.

Abstract: Disclosed herein is an artificial transmembrane protein for use in a biomolecular detection device for detecting intracellular or intravesicular biomolecular interactions, the artificial transmembrane protein having an extracellular or extravesicular binder structure, a hydrophobic transmembrane domain, and an intracellular or intravesicular domain with an intracellular or intravesicular receptor structure, wherein the receptor structure is configured to interact with an intracellular or intravesicular component of the biomolecular interaction to be detected and wherein the extracellular or extravesicular binder structure is configured to bind to membrane recognition elements arranged along a plurality of predetermined lines of the biomolecular detection device.

Abstract: Methods and devices for electrochemically controlled degradation of metals particles using halide biochemistry allowing in-situ control and being suitable for analyte detection and quantification.

Abstract: A diffractometric sensing device for analyzing molecular interactions is described, the diffractometric sensing device comprising a transparent carrier medium, the carrier medium comprising a grating structure (42.1-42.5) with a plurality of consecutive surfaces or lines and a plurality of binding sites arranged thereon, the binding sites being configured to interact with one or more target molecules, wherein the grating structure is configured to diffract a portion of coherent light propagating in the carrier medium so as to produce a constructive interference signal at a light detector (4.1-4.4), the signal being dependent on molecular interactions at or in the vicinity of the binding sites. The invention offers more sensitivity, faster response, miniaturization, easy manufacturing and more applications compared to current diffractometric sensing devices.

Abstract: Disclosed herein is a biomolecular detection device (1) for analyzing a cell, vesicle or a cellular or vesicular component, comprising an evanescent illuminator with an optical coupling unit configured for generating an evanescent field from coherent light (L) with a predefined wavelength on a first surface of the evanescent illuminator. The first surface of evanescent illuminator comprises a template nanopattern (5), containing a coherent arrangement of a plurality of predetermined lines along which membrane recognition elements for a binder structure (82) of a transmembrane protein (81), preferably a laterally diffusible transmembrane protein, of the cell, vesicle or the cellular or vesicular component (8) are arranged.

Abstract: A method for manufacturing a three-dimensional object comprises the steps of (a) bringing at least one nozzle in a first position close to a surface of a substrate, (b) delivering through said at least one nozzle at least one reactant to said surface, (c) effecting a solid forming reaction of said at least one delivered reactant such that said at least one delivered reactant undergoes a transition to become a growing solid deposit on said surface under said at least one nozzle, and (d) detecting an interaction of said growing solid deposit with said at least one nozzle.

Abstract: The invention relates to a device (1), a method, and a kit for analysing liquid samples. The device (1) comprises a sample layer (111) having a plurality of liquid permeable test sites (112) separated by a liquid impermeable barrier region (113), and an inlet part (2) comprising a plurality of inlet channels (211), which lead to respective test sites (112) of the sample layer (111), such that a flow connection between said inlet channels (211) and said respective test sites (112) is established or can be established, wherein said inlet channels (211) comprise first openings (218) and second openings (219), wherein a second surface area defined by the positions of said second openings (219) is smaller than a first surface area defined by the positions of said first openings (218) The invention further relates to a method for functionalizing a sample layer (111).

Abstract: A method for manufacturing a three-dimensional object comprises the steps of (a) bringing at least one nozzle in a first position close to a surface of a substrate, (b) delivering through said at least one nozzle at least one reactant to said surface, (c) effecting a solid forming reaction of said at least one delivered reactant such that said at least one delivered reactant undergoes a transition to become a growing solid deposit on said surface under said at least one nozzle, and (d) detecting an interaction of said growing solid deposit with said at least one nozzle.

Abstract: The invention relates to a device (1), a method, and a kit for analysing liquid samples. The device (1) comprises a sample layer (111) having a plurality of liquid permeable test sites (112) separated by a liquid impermeable barrier region (113), and an inlet part (2) comprising a plurality of inlet channels (211), which lead to respective test sites (112) of the sample layer (111), such that a flow connection between said inlet channels (211) and said respective test sites (112) is established or can be established, wherein said inlet channels (211) comprise first openings (218) and second openings (219), wherein a second surface area defined by the positions of said second openings (219) is smaller than a first surface area defined by the positions of said first openings (218) The invention further relates to a method for functionalizing a sample layer (111).

Abstract: Small and extremely small molecules and ions or atoms may be detected with the novel device with exceptional sensitivity. The detection is implemented in a simple manner by the known acoustic resonator FBAR or by means of other technologies that measure the physical properties of the filled layer. The permeability of substances (e.g. active ingredients) through membranes such as cell membranes, lipid bilayers, and cell walls can be examined by combining a sensor with the reservoir and the membrane.

Abstract: The invention relates to a method for spatially manipulating a microscopic object including providing a cantilever (12) having a tip with an opening (19) and a microchannel (15) extending through the cantilever (12) in its longitudinal direction. A suspension means is provided for holding the cantilever (12) and spatially moving the cantilever along a predetermined spatial path. A pressurizing means is provided for applying a predetermined pressure to the microchannel (15) and the cantilever (12) is moved with its tip to the microscopic object to be spatially manipulated. At least a part of the microscopic object is picked up with said cantilever (12) by reducing the pressure within the microchannel (15) relative to the pressure outside the tip of the cantilever (12). The microscopic object is then moved along a predetermined spatial path by means of the cantilever (12).

Abstract: The invention relates to a probe arrangement (10g) for exchanging in a controllable way liquids with micro-sized samples of material like biological cells, especially in connection with an scanning probe microscope, said probe arrangement (10g) comprising a probe holder (11) with at least one embedded first channel (18) and a cantilever (12) with at least one embedded second channel (15) and being provided to be attached to said probe holder (11) in a way that at least one aperture (19) of the first channel (18) is connected to at least one aperture (17) of the second channel (15) in a way that permits the liquid-tight transfer of a liquid between said first and second channels (15, 18). A safe and easy use of the probe arrangement is achieved by having the cantilever (12) permanently attached to said probe holder (11) to form a prefabricated probe unit (10g).

Abstract: A hopper having a passage through which a medicine can be passed downward is provided. A part of a lower portion of the hopper is a deformable portion having flexibility, and the deformable portion is deformable so as to open and close the passage. According to this configuration, the deformable portion that is a part of the hopper is deformed to open and close the passage. Thus, there is no portion such as an opening and closing plate on which the medicine remains, thereby preventing a gap in which the medicine remains from being formed in the hopper, and preventing the medicine from easily remaining in the hopper.

Abstract: The present invention relates to a bioanalytical device consisting of a microwell array with microwell (2) that are filled with assay components (12, 15, 36), wherein detection probes (36) used in the assay (10) are metal nanoparticles (11, 12) or fluorescent compounds, and wherein the microwell array is connected and/or connectable to a sample that is on a flat substrate (6) to quantify the amount of a ligand (35) in the sample by using a detection mechanism. The detection mechanism is based on change in the optical properties of some of the assay components (12, 15, 36) upon contact with the ligand (35). The present invention also relates further to a method for detecting and quantifying molecules using said bioanalytical device.

Abstract: The invention relates to a method for spatially manipulating a microscopic object (20,23,33), said method comprising the steps of providing a cantilever (12) having a tip with an opening (19) and a microchannel (15) extending through the cantilever (12) in its longitudinal direction, said microchannel (15) being fluidly connected to the opening (19) at the tip of the cantilever; providing suspension means for holding the cantilever (12) and spatially moving the cantilever along a predetermined spatial path; providing pressurizing means for applying a predetermined pressure to the microchannel (15) within the cantilever; moving the cantilever (12) with its tip to the microscopic object (20,23,33) to be spatially manipulated, such that the opening (19) of the tip is adjacent to the microscopic object (20,23,33); picking up, with said cantilever (12), a part of the microscopic object (20,23,33) or the microscopic object (20,23,33) as a whole by reducing the pressure within the microchannel (15) relative to the p

Abstract: A method for measuring transport properties of cell membranes or lipid bilayers by providing at least a substrate having a topside and a backside and plurality of nano- or micro-pores and a cell membrane covering the plurality of pores being accessible from both sides of the cell membrane for measurement, a material layer at least in the region of the pores to support the cell membrane at the pores but not hindering the transport through the cell membrane and the pores arranged on the substrate, either on the topside or on the backside, applying a fluid containing at least one molecule to the topside of the membrane in order to allow the molecule to move through the membrane, one of the pores and the material layer, and monitoring of the molecules having passed the material layer by using an optical detection method.

Abstract: Small and extremely small molecules and ions or atoms may be detected with the novel device with exceptional sensitivity. The detection is implemented in a simple manner by the known acoustic resonator FBAR or by means of other technologies that measure the physical properties of the filled layer. The permeability of substances (e.g. active ingredients) through membranes such as cell membranes, lipid bilayers, and cell walls can be examined by combining a sensor with the reservoir and the membrane.

Abstract: The invention relates to a probe arrangement (10g) for exchanging in a controllable way liquids with micro-sized samples of material like biological cells, especially in connection with an scanning probe microscope, said probe arrangement (10g) comprising a probe holder (11) with at least one embedded first channel (18) and a cantilever (12) with at least one embedded second channel (15) and being provided to be attached to said probe holder (11) in a way that at least one aperture (19) of the first channel (18) is connected to at least one aperture (17) of the second channel (15) in a way that permits the liquid-tight transfer of a liquid between said first and second channels (15, 18). A safe and easy use of the probe arrangement is achieved by having the cantilever (12) permanently attached to said probe holder (11) to form a prefabricated probe unit (10g).

Abstract: A probe arrangement with a probe for local electrophysiological analysis of cells (4) such as patch-clamp techniques for use with atomic force microscopy, has a probe with a cantilever arm (2) connected to a probe holder (3). The probe has a probe tip (4) at a probing end (5) of the cantilever arm (2) and a fluid channel (6) in the cantilever arm (2) connecting a probe tip aperture (7) with a fluid reservoir (8) via a duct (9). The fluid channel (6), the duct (9) and the fluid reservoir (8) are adapted to be filled with a fluid solution (10) enabling ion transport for electrophysiological analysis. A first electrode (15) for electrophysiological analysis is placed in the fluid reservoir (8) and/or in the duct (9) and/or in the fluid channel (6).