Abstract: Described herein are methods of purifying polynucleotides, e.g., imRNA and oligonucleotides, e.g., probes, primers and siRNA, using monolithic columns with immobilized ligands coupled to the monolithic column. Also described are monolithic columns for purifying polynucleotides from a sample; and methods of preparing such columns.

Abstract: A recessed luminaire includes a trim, a junction box, and a light source socket. The trim includes a trim body, a flange, and a cover section. The flange extends out from the trim body at an opening of the trim. The junction box and the light source socket are attached to the trim. The light source socket is positioned inside a cavity of the trim. The light source socket may be attached to a cover section of the trim or to a bracket that is attached to the trim.

Abstract: A retrofitted light fixture can include an existing power supply of an existing light fixture that receives, when in a retrofitted state, AC mains power directly from a power source and delivers primary power to one or more existing light fixture components of the existing light fixture. The retrofitted light fixture can also include an adapter coupled to and disposed between the existing power supply and the power source, where the adapter provides the AC mains power to the power supply, where the adapter includes at least one retrofit component lacking from the existing light fixture, where the at least one retrofit component comprises a controller and a transceiver.

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

Abstract: Techniques related to vulnerability assessment based on machine inference are disclosed. A vulnerability assessment server may receive, from a client device, a set of metadata corresponding to a program stored on the client device. Further, the vulnerability assessment server may extract a program name from the set of metadata. Still further, the vulnerability assessment server may determine one or more vulnerabilities of the program based on searching for the program name in one or more storage systems that maintain sets of vulnerability data.

Abstract: Techniques related to vulnerability assessment based on machine inference are disclosed. A vulnerability assessment server may receive, from a client device, a set of metadata corresponding to a program stored on the client device. Further, the vulnerability assessment server may extract a program name from the set of metadata. Still further, the vulnerability assessment server may determine one or more vulnerabilities of the program based on searching for the program name in one or more storage systems that maintain sets of vulnerability data.

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

Abstract: This invention provides expressible polynucleotides, which can express a target protein or polypeptide. Synthetic mRNA constructs for producing a protein or polypeptide can contain one or more 5? UTRs, where a 5? UTR may be expressed by a gene of a plant. In some embodiments, a 5? UTR may be expressed by a gene of a member of Arabidopsis genus. The synthetic mRNA constructs can be used as pharmaceutical agents for expressing a target protein or polypeptide in vivo.

Abstract: A recessed luminaire includes a trim, a junction box, and a light source socket. The trim includes a trim body, a flange, and a cover section. The flange extends out from the trim body at an opening of the trim. The junction box and the light source socket are attached to the trim. The light source socket is positioned inside a cavity of the trim. The light source socket may be attached to a cover section of the trim or to a bracket that is attached to the trim.

Abstract: This invention provides expressible polynucleotides, which can express a target protein or polypeptide. Synthetic mRNA constructs for producing a protein or polypeptide can contain one or more 5? UTRs, where a 5? UTR may be expressed by a gene of a plant. In some embodiments, a 5? UTR may be expressed by a gene of a member of Arabidopsis genus. The synthetic mRNA constructs can be used as pharmaceutical agents for expressing a target protein or polypeptide in vivo.

Abstract: The disclosure provides a method of producing recombinant alkaline phosphatase comprising: (i) culturing an alkaline phosphatase in a recombinant cell culture; (ii) obtaining a preparation of recombinant alkaline phosphatase from the cell culture; and (iii) minimizing in the preparation a concentration of at least one metal ion selected from the group consisting of: Nickel (Ni), Cobalt (Co), Copper (Cu), Manganese (Mn), Chromium (Cr), and Molybdenum (Mo).

Abstract: An adjustable light fixture can include a housing having at least one wall that forms a first cavity, wherein in the housing has a top portion and a bottom end. The fixture can also further include a body having a first body portion and a second body portion, where the body is movably disposed within the first cavity of the housing, where the body is configured to have a plurality of positions relative to the housing. The adjustable light fixture can also include a resilient device disposed between the first body portion and the second body portion to simultaneously push the first body portion against the top portion of the housing and the second body portion against the bottom end of the housing when the body is in each of the positions relative to the housing.

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

Abstract: An adjustable light fixture can include a housing comprising at least one wall that forms a first cavity. The adjustable light fixture can also include a first sealing member coupled to the at least one wall and at least partially disposed within the first cavity. The adjustable light fixture can further include a body movably coupled to the housing and at least partially disposed within the first cavity, where the body is configured to have multiple positions relative to the housing, where the first sealing member abuts against an outer surface of the body when the body is in each of the positions, where the first sealing member prevents fluids in an ambient environment from traversing therethrough when the body is in each of the positions, where the positions includes the body having a tilt angle relative to the housing of greater than 20°.

Abstract: The disclosure provides a method of producing recombinant alkaline phosphatase comprising: (i) culturing an alkaline phosphatase in a recombinant cell culture; (ii) obtaining a preparation of recombinant alkaline phosphatase from the cell culture; and (iii) minimizing in the preparation a concentration of at least one metal ion selected from the group consisting of: Nickel (Ni), Cobalt (Co), Copper (Cu), Manganese (Mn), Chromium (Cr), and Molybdenum (Mo).

Abstract: An adjustable light fixture can include a housing comprising at least one wall that forms a first cavity. The adjustable light fixture can also include a first sealing member coupled to the at least one wall and at least partially disposed within the first cavity. The adjustable light fixture can further include a body movably coupled to the housing and at least partially disposed within the first cavity, where the body is configured to have multiple positions relative to the housing, where the first sealing member abuts against an outer surface of the body when the body is in each of the positions, where the first sealing member prevents fluids in an ambient environment from traversing therethrough when the body is in each of the positions, where the positions includes the body having a tilt angle relative to the housing of greater than 20°.

Abstract: Techniques related to vulnerability assessment based on machine inference are disclosed. A vulnerability assessment server may receive, from a client device, a set of metadata corresponding to a program stored on the client device. Further, the vulnerability assessment server may extract a program name from the set of metadata. Still further, the vulnerability assessment server may determine one or more vulnerabilities of the program based on searching for the program name in one or more storage systems that maintain sets of vulnerability data.