Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic coronavirus proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.

Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.

Abstract: A location awareness system including a communication network, and a network operating element coupled to the communication network. At least one anchor network gateway is coupled to the communication network, the at least one anchor network gateway configured to generate a wireless anchor network. A plurality of anchors are configured to couple to one of the at least one anchor network gateway via its respective wireless anchor network. A plurality of tags is each configured to communicate with at least one anchor to provide ranging information for determination of a position of the tag within an area covered by the system.

Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.

Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic coronavirus proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.

Abstract: Provided herein are methods for purification of RNA from a sample. The methods include obtaining a first sample including double stranded RNA in a loading buffer, loading the sample onto a ceramic hydroxyapatite column, washing the column with wash buffer, and eluting the column with an elution buffer to create an eluate.

Abstract: The disclosed bag for mixing materials may include a front wall, a back wall, a mixing chamber between the front wall and the back wall, a first sidewall between the front wall and the back wall and defining a first side of the mixing chamber, a second sidewall between the front wall and the back wall defining a second, opposite side of the mixing chamber, and a port positioned to provide fluid access to the mixing chamber from a bottom of the mixing chamber, wherein the first sidewall and the second sidewall are shaped to alternate a direction of fluid flow when fluid is introduced into the mixing chamber through the port. Various other components, systems, and methods are also disclosed.

Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.

Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic coronavirus proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.

Abstract: A beverage temperature-monitoring device that is comprised of a supporting member that adapted to mount on rim of the container that holds the beverage, a heat sensor coupled to inner arm of the supporting member and is in continuous contact with the beverage, a dashboard coupled to outer arm of supporting member and operably coupled to the heat sensor. The dashboard includes an integrated display screen to show the actual and desired temperature set by user and have input means to set the desired temperature. The dashboard further includes the audio and visual indicators to alert the user when the temperature of the beverage reaches the desired temperature.

Abstract: A self-cleaning, low-maintenance, water-saving automated animal watering device (10) is provided which includes a watering tank (12) moveable between a lower, water-full condition and an upper, water-low condition. Preferably, one end of the tank (12) is pivotally supported by a pivot shaft (64), while the other end of the tank is biased upwardly via a lower lift spring (72). The device (10) also has a water fill assembly (16) operably coupled with tank (12) to selectively fill the tank (12) when needed, and a drain valve assembly (18) also coupled with tank (12) in order to completely drain the tank (12) on a periodic basis. A control assembly (20) is coupled with at least the tank (12) and drain valve assembly (18), and is operable to actuate the drain valve assembly (18) after a predetermined number of movements of the tank (12) between the elevated and lowered positions thereof.

Abstract: A self-cleaning, low-maintenance, water-saving automated animal watering device (10) is provided which includes a watering tank (12) moveable between a lower, water-full condition and an upper, water-low condition. Preferably, one end of the tank (12) is pivotally supported by a pivot shaft (64), while the other end of the tank is biased upwardly via a lower lift spring (72). The device (10) also has a water fill assembly (16) operably coupled with tank (12) to selectively fill the tank (12) when needed, and a drain valve assembly (18) also coupled with tank (12) in order to completely drain the tank (12) on a periodic basis. A control assembly (20) is coupled with at least the tank (12) and drain valve assembly (18), and is operable to actuate the drain valve assembly (18) after a predetermined number of movements of the tank (12) between the elevated and lowered positions thereof.