Patents by Inventor Jared Leadbetter

Jared Leadbetter has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10328428
    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.
    Type: Grant
    Filed: January 13, 2017
    Date of Patent: June 25, 2019
    Assignee: California Institute of Technology
    Inventors: Jong Wook Hong, Vincent Studer, W. French Anderson, Stephen R. Quake, Jared Leadbetter
  • Publication number: 20190009272
    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.
    Type: Application
    Filed: January 13, 2017
    Publication date: January 10, 2019
    Inventors: Jong Wook Hong, Vincent Studer, W. French Anderson, Stephen R. Quake, Jared Leadbetter
  • Patent number: 9579650
    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.
    Type: Grant
    Filed: September 23, 2014
    Date of Patent: February 28, 2017
    Assignee: California Institute of Technology
    Inventors: Jong Wook Hong, Vincent Studer, W. French Anderson, Stephen R. Quake, Jared Leadbetter
  • Publication number: 20150238960
    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.
    Type: Application
    Filed: September 23, 2014
    Publication date: August 27, 2015
    Inventors: Jong Wook Hong, Vincent Studer, W. French Anderson, Stephen R. Quake, Jared Leadbetter
  • Patent number: 8871446
    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.
    Type: Grant
    Filed: October 2, 2003
    Date of Patent: October 28, 2014
    Assignee: California Institute of Technology
    Inventors: Jong Wook Hong, Vincent Studer, W. French Anderson, Stephen R. Quake, Jared Leadbetter
  • Patent number: 8551725
    Abstract: Screening assays that allow for the identification of agents that increase acyl homoserine lactone (AHL) acylase expression and/or AHL acylase activity in ?-proteobacteria such as Pseudomonas aeruginosa. Such agents are useful, for example, for inhibiting quorum sensing activity of such bacteria by increasing degradation of long chain, but not short chain, AHLs and, therefore, can be useful for treating infections by such bacteria.
    Type: Grant
    Filed: January 24, 2008
    Date of Patent: October 8, 2013
    Assignee: California Institute of Technology
    Inventors: Jared Leadbetter, Jean J. Huang
  • Publication number: 20080220978
    Abstract: Screening assays that allow for the identification of agents that increase acyl homoserine lactone (AHL) acylase expression and/or AHL acylase activity in ?-proteobacteria such as Pseudomonas aeruginosa. Such agents are useful, for example, for inhibiting quorum sensing activity of such bacteria by increasing degradation of long chain, but not short chain, AHLs and, therefore, can be useful for treating infections by such bacteria.
    Type: Application
    Filed: January 24, 2008
    Publication date: September 11, 2008
    Inventors: Jared Leadbetter, Jean J. Huang
  • Patent number: 7335352
    Abstract: Screening assays that allow for the identification of agents that increase acyl homoserine lactone (AHL) acylase expression and/or AHL acylase activity in ?-proteobacteria such as Pseudomonas aeruginosa. Such agents are useful, for example, for inhibiting quorum sensing activity of such bacteria by increasing degradation of long chain, but not short chain, AHLs and, therefore, can be useful for treating infections by such bacteria.
    Type: Grant
    Filed: June 3, 2004
    Date of Patent: February 26, 2008
    Assignee: California Institute of Technology
    Inventors: Jared Leadbetter, Jean J. Huang
  • Publication number: 20050053952
    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.
    Type: Application
    Filed: October 2, 2003
    Publication date: March 10, 2005
    Applicant: California Institute of Technology
    Inventors: Jong Hong, Vincent Studer, W. Anderson, Stephen Quake, Jared Leadbetter