Abstract: Systems and processes for recovering a condensate from a conduit. The system can include a buoy that can include a fluid swivel assembly. The system can also include a floating conduit and first and second gas transfer conduits that can be in fluid communication with the fluid swivel assembly and configured to transfer a gas discharged from a vessel storage tank to a gas pipeline. The system can also include first and second liquid transfer conduits in fluid communication with the fluid swivel assembly and configured to transfer a liquid from a liquid pipeline to the vessel storage tank. The system can also include a condensation conduit configured to transfer at least a portion of any condensate that accumulates within an internal volume of the floating conduit into a fluid flow path defined by the fluid swivel assembly, the second liquid transfer conduit, or the first liquid transfer conduit.

Abstract: An additive manufactured piston having a crown portion and a skirt portion form of an aluminum-cerium-silicon alloy. The piston includes a least one recess pocket in an unstressed region located at an interface between an inner surface of the crown portion and an outer surface of the skirt portion. The skirt portion includes an outer panel surface defining an external rib adjacent a peripheral end surface, at least one stiffening rib extending from a pin boss to a skirt end, and a wall thickness of between 0.2 mm and 1.0 mm in a region between the external rib and the at least one stiffening rib. The skirt portion further includes an internal first rib having a width W1 on a thrust side portion and an internal second rib having a width W2 located on an anti-thrust side portion, in which W1 is greater than W2.

Abstract: Described herein are methods of modifying a target gene in a patient or in a patient-derived cell, wherein the patient has an autosomal recessive disorder with compound heterozygous mutations, the methods including delivering a first modified guide RNA, a second modified guide RNA, a Cas9 polypeptide, a biotin-binding molecule, a first biotinylated donor polynucleotide, and a second biotinylated donor polynucleotide. The first modified guide RNA and the first biotinylated donor polynucleotide correct a first diseased allele, and the second modified guide RNA and the second biotinylated donor polynucleotide correct a second diseased allele.

Abstract: An additive manufactured piston having a crown portion and a skirt portion form of an aluminum-cerium-silicon alloy. The piston includes a least one recess pocket in an unstressed region located at an interface between an inner surface of the crown portion and an outer surface of the skirt portion. The skirt portion includes an outer panel surface defining an external rib adjacent a peripheral end surface, at least one stiffening rib extending from a pin boss to a skirt end, and a wall thickness of between 0.2 mm and 1.0 mm in a region between the external rib and the at least one stiffening rib. The skirt portion further includes an internal first rib having a width W1 on a thrust side portion and an internal second rib having a width W2 located on an anti-thrust side portion, in which W1 is greater than W2.

Abstract: A computer-implemented method of mapping a process model of a software application executed by a hosting platform. A series of actions taken by a user with respect to user interfaces generated by the software application are simulated through one or more application programming interface (API) calls. The user interfaces include a form and the series of actions includes opening the form. A plurality of user interface fields of the form are then identified through one or more other API calls executed while impersonating a session of a user under test. At least some of the user interface fields are set to known values and the form is submitted to the software application after the fields have been set. Changes to field-related and record-related information resulting from the submission are then gathered and a process model of the software application is determined based upon the gathered information.

Abstract: Described herein are guide RNAs and modified guide RNAs suitable for biallelic correction of Pompe disease. Also included are methods of modifying a target gene in a patient or in a patient-derived cell, wherein the patient has an autosomal recessive disorder with compound heterozygous mutations, the methods including delivering a first modified guide RNA, a second modified guide RNA, a Cas9 polypeptide, a biotin-binding molecule, a first biotinylated donor polynucleotide, and a second biotinylated donor polynucleotide. The first modified guide RNA and the first biotinylated donor polynucleotide correct a first diseased allele, and the second modified guide RNA and the second biotinylated donor polynucleotide correct a second diseased allele.

Abstract: A heat exchanger and heat exchanger core are provided. The heat exchanger core includes a plurality of columnar passages extending between an inlet plenum of the heat exchanger core and an outlet plenum of the heat exchanger core, the columnar passages formed monolithically in a single fabrication process.

Abstract: A computer-implemented method of mapping a process model of a software application executed by a hosting platform. A series of actions taken by a user with respect to user interfaces generated by the software application are simulated through one or more application programming interface (API) calls. The user interfaces include a form and the series of actions includes opening the form. A plurality of user interface fields of the form are then identified through one or more other API calls executed while impersonating a session of a user under test. At least some of the user interface fields are set to known values and the form is submitted to the software application after the fields have been set. Changes to field-related and record-related information resulting from the submission are then gathered and a process model of the software application is determined based upon the gathered information.

Abstract: A computer-implemented method of mapping a process model of a software application executed by a hosting platform. A series of actions taken by a user with respect to user interfaces generated by the software application are simulated through one or more application programming interface (API) calls. The user interfaces include a form and the series of actions includes opening the form. A plurality of user interface fields of the form are then identified through one or more other API calls executed while impersonating a session of a user under test. At least some of the user interface fields are set to known values and the form is submitted to the software application after the fields have been set. Changes to field-related and record-related information resulting from the submission are then gathered and a process model of the software application is determined based upon the gathered information.

Abstract: A storage system includes a floor; a pair of end walls hingedly and detachably affixable to the floor; a pair of side walls hingedly and detachably affixable to the floor; and a cover hingedly and detachably affixable to one of the end walls.

Abstract: A integrated fluid conduit is provided having two or more internal conduits, the size and shape of the internal conduits is designed to optimize the hydraulic diameter of each conduit based on the needs of the system. The integrated fluid conduit is advantageously manufactured according to additive manufacturing techniques which enable formation of off-center and/or non-concentric internal conduits adapted to specific applications requiring turns such as the fuel delivery system or the hydraulic system of/on a gas turbine engine for an airplane.

Abstract: A heat exchanger and heat exchanger core are provided. The heat exchanger core includes a plurality of columnar passages extending between an inlet plenum of the heat exchanger core and an outlet plenum of the heat exchanger core, the columnar passages formed monolithically in a single fabrication process.

Abstract: Described herein are modified guide RNAs such as a single guide RNA including, from 5? to 3?, a single-stranded protospacer sequence, a first complementary strand of a binding region for the Cas9 polypeptide, an aptamer that binds a biotin-binding molecule, and a second complementary strand of the binding region for the Cas9 polypeptide. Also described is an RNP complex including the modified guide RNA and a Cas9 polypeptide or active fragment thereof. Also included are methods of modifying target genes in cells using the modified guide RNAs.

Abstract: The present application is directed to a system for collecting or extruding a cell-containing biological material. The system includes an extrusion/collection device comprising: an orifice and a chamber operably connected to the orifice and an electrical impedance spectroscopy (EIS) device, operably connected to the extrusion/collection device. The EIS device monitors biologically relevant attributes of a cell-containing biological material as it is extruded from or collected into the extrusion/collection device. Also disclosed are methods of using the system disclosed herein.

Abstract: Described herein are modified guide RNAs such as a single guide RNA including, from 5? to 3?, a single-stranded protospacer sequence, a first complementary strand of a binding region for the Cas9 polypeptide, an aptamer that binds a biotin-binding molecule, and a second complementary strand of the binding region for the Cas9 polypeptide. Also described is an RNP complex including the modified guide RNA and a Cas9 polypeptide or active fragment thereof. Also included are methods of modifying target genes in cells using the modified guide RNAs.

Abstract: This disclosure describes a transportation matching system that utilizes one or more balancer models to generate an electronic communication distribution strategy based on relative impacts of provider-specific and requester-specific levers over a target time horizon. The disclosed systems utilize the balancer models to generate predictive functions for providers and requesters to determine lever content to distribute (e.g., within electronic communications) to providers and/or requesters to efficiently and/or effectively produce acquisition and/or engagement for a target time horizon. Based on the predictive functions, the disclosed systems generate an electronic communication distribution strategy to provide (or cause to be provided) electronic communications to providers and requesters to efficiently and effectively increase or decrease acquisition and/or engagement.

Abstract: A heat exchanger is provided having an integrally and seamlessly formed return manifold connecting multiple supply tubes and return tubes. The heat exchanger may also include a return manifold having one or more structures providing a flow restriction within or proximate the return manifold.

Abstract: A heat exchanger and heat exchanger core are provided. The heat exchanger core includes a plurality of columnar passages extending between an inlet plenum of the heat exchanger core and an outlet plenum of the heat exchanger core, the columnar passages formed monolithically in a single fabrication process.

Abstract: A integrated fluid conduit is provided having two or more internal conduits, the size and shape of the internal conduits is designed to optimize the hydraulic diameter of each conduit based on the needs of the system. The integrated fluid conduit is advantageously manufactured according to additive manufacturing techniques which enable formation of off-center and/or non-concentric internal conduits adapted to specific applications requiring turns such as the fuel delivery system or the hydraulic system of/on a gas turbine engine for an airplane.

Abstract: A computer-implemented method of mapping a process model of a software application executed by a hosting platform. A series of actions taken by a user with respect to user interfaces generated by the software application are simulated through one or more application programming interface (API) calls. The user interfaces include a form and the series of actions includes opening the form. A plurality of user interface fields of the form are then identified through one or more other API calls executed while impersonating a session of a user under test. At least some of the user interface fields are set to known values and the form is submitted to the software application after the fields have been set. Changes to field-related and record-related information resulting from the submission are then gathered and a process model of the software application is determined based upon the gathered information.