Abstract: The present disclosure relates to methods for detecting unique genetic signatures derived from markers such as, for example, mutations, somatic or germ-line, in nucleic acids obtained from biological samples. The sensitivity of the methods provides for detection of mutations associated with a disease, e.g., cancer mutations, or with inherited disease, e.g., an autosomal recessive disease, in a noninvasive manner at ultra-low proportions of sequences carrying mutations to sequences carrying normal, e.g., non-cancer sequences, or a reference sequence, e.g., a human reference genome.

Abstract: A method for generating a structurally altered gas molecule from water. An example method includes placing an electrolyte solution in a chemical reaction chamber, adding purified water to the chemical reaction chamber, and applying a focused magnetic field and an electric field to a mixture of the purified water and the electrolyte solution to cause generation of the structurally altered gas molecule from the purified water. The structurally altered gas molecule is a combination of two parts of hydrogen and one part of oxygen. The structurally altered gas molecule has a hydrogen-oxygen-hydrogen bond angle between 94 degrees and 104 degrees and hydrogen-oxygen bond length between 0.95 Angstrom and 1.3 Angstrom. The structurally altered gas molecule is stable at a pressure exceeding 300 pounds per square inch gauge.

Abstract: The present disclosure relates to methods for detecting unique genetic signatures derived from markers such as, for example, mutations, somatic or germ-line, in nucleic acids obtained from biological samples. The sensitivity of the methods provides for detection of mutations associated with a disease, e.g., cancer mutations, or with inherited disease, e.g., an autosomal recessive disease, in a noninvasive manner at ultra-low proportions of sequences carrying mutations to sequences carrying normal, e.g., non-cancer sequences, or a reference sequence, e.g., a human reference genome.

Abstract: A printer that forms a three-dimensional object includes a single arm with all 3 axes on the head to move spatially while a substrate underneath the head remains fixed. Improved design and components enhance 3D movement, access to components, performance, durability, and ease of service.

Abstract: The present disclosure relates to methods for detecting unique genetic signatures derived from markers such as, for example, mutations, somatic or germ-line, in nucleic acids obtained from biological samples. The sensitivity of the methods provides for detection of mutations associated with a disease, e.g., cancer mutations, or with inherited disease, e.g., an autosomal recessive disease, in a noninvasive manner at ultra-low proportions of sequences carrying mutations to sequences carrying normal, e.g., non-cancer sequences, or a reference sequence, e.g., a human reference genome.

Abstract: The present disclosure relates to methods for detecting unique genetic signatures derived from markers such as, for example, mutations, somatic or germ-line, in nucleic acids obtained from biological samples. The sensitivity of the methods provides for detection of mutations associated with a disease, e.g., cancer mutations, or with inherited disease, e.g., an autosomal recessive disease, in a noninvasive manner at ultra-low proportions of sequences carrying mutations to sequences carrying normal, e.g., non-cancer sequences, or a reference sequence, e.g., a human reference genome.

Abstract: A printer that forms a three-dimensional object includes a single arm with all 3 axes on the head to move spatially while a substrate underneath the head remains fixed. Improved design and components enhance 3D movement, access to components, performance, durability, and ease of service.

Abstract: Capture probe libraries can be used to enrich a region of interest in a sequencing library for high-depth sequencing. The capture probes within the capture probe libraries often do not function in a predictable or uniform manner. Described herein are balanced capture probe libraries and methods of balancing capture probe libraries. A sequencing library can be enriched using balanced capture probe libraries, and the enriched sequencing library can be sequenced to obtain a sequencing depth closer to a desired sequencing depth.

Abstract: Prenatal genetic testing allows early detection of genetic disease in a fetus. Described herein are methods of detecting the presence or absence of a genetic variant in a region of interest in the genome of a fetus in a pregnant woman. The methods are noninvasive, and can use cell-free DNA (cfDNA) present in the plasma of the pregnant woman. A DNA library is constructed from the cfDNA, and DNA molecules comprising the region of interest or portions thereof are enriched and analyzed, for example by sequencing. The methods described herein can also rely on constructing a maternal haplotype to provide even higher resolution fetal genetic variant determination.

Abstract: The present disclosure relates to methods for detecting unique genetic signatures derived from markers such as, for example, mutations, somatic or germ-line, in nucleic acids obtained from biological samples. The sensitivity of the methods provides for detection of mutations associated with a disease, e.g., cancer mutations, or with inherited disease, e.g., an autosomal recessive disease, in a noninvasive manner at ultra-low proportions of sequences carrying mutations to sequences carrying normal, e.g., non-cancer sequences, or a reference sequence, e.g., a human reference genome.

Abstract: Processes and systems for reading variants from a genome sample relative to a reference genomic sequence are provided. An exemplary process includes collecting a set reads and generating a k-mer graph from the reads. For example, the k-mer graph can be constructed to represent all possible substrings of the collected reads. The k-mer graph may be reduced to a contiguous graph, and a set of possible haplotypes generated from the contiguous graph. The process may further generate, the error table providing a filter for common sequencer errors. The process may then generate a set of diplotypes based on the set of haplotypes and the generated error table and score the set of diplotypes to identify variants from the reference genome. Scoring the diplotypes may include determining a posterior probability for each of the diplotypes, with the highest scoring diplotype(s) reported as the result.

Abstract: The present invention extends to methods, systems, and computer program products for linking diagnostic visualizations to regions of application code. Diagnostic visualizations emitted during execution of an application are displayed. The diagnostic visualizations partially represent the abstract objective of the application (e.g., as envisioned by a developer). Diagnostic data for at least one of a plurality of components is displayed. The diagnostic data indicates the performance of the at least one of the plurality of components during execution of the application. The displayed one or more diagnostic visualizations and the displayed diagnostic data is correlated to link the one or more diagnostic visualizations to the at least one of the plurality of components. Linking the one or more diagnostic visualizations to the at least one of the plurality of components can better indicate how the application's behavior reconciles the abstract objective.

Abstract: An embed code that can be copied from a first web site for placement in a second web site by users is disclosed. The embed code will render a content object from the second web site on a number of supported platforms. Autonomously, the embed code chooses from multiple playback options to choose one for the web browser rendering the embed code, for example, Flash,™ HTML5, Silverlight,™ or a media player. Wherever the embed code is placed in a web site, a wide range of end user devices are supported without needing to reference other locations on the Internet, i.e., the embed code alone can automatically configure to play on that wide range of end user devices. The content object may be hosted or stored in various locations with the embed code accessing the proper version of the end user device.

Abstract: The present invention extends to methods, systems, and computer program products for linking diagnostic visualizations to regions of application code. Diagnostic visualizations emitted during execution of an application are displayed. The diagnostic visualizations partially represent the abstract objective of the application (e.g., as envisioned by a developer). Diagnostic data for at least one of a plurality of components is displayed. The diagnostic data indicates the performance of the at least one of the plurality of components during execution of the application. The displayed one or more diagnostic visualizations and the displayed diagnostic data is correlated to link the one or more diagnostic visualizations to the at least one of the plurality of components. Linking the one or more diagnostic visualizations to the at least one of the plurality of components can better indicate how the application's behavior reconciles the abstract objective.

Abstract: An embed code that can be copied from a first web site for placement in a second web site by users is disclosed. The embed code will render a content object from the second web site on a number of supported platforms. Autonomously, the embed code chooses from multiple playback options to choose one for the web browser rendering the embed code, for example, Flash,™ HTML5, Silverlight,™ or a media player. Wherever the embed code is placed in a web site, a wide range of end user devices are supported without needing to reference other locations on the Internet, i.e., the embed code alone can automatically configure to play on that wide range of end user devices. The content object may be hosted or stored in various locations with the embed code accessing the proper version of the end user device.

Abstract: An exercise apparatus which may be moveable between a storage mode and an exercise mode. The exercise apparatus may used on a base surface by a user. The exercise apparatus may include a frame for supporting a seat above the base surface. The frame may include a front leg. The frame may include an engaging member that may be pivotally connected to the front leg comprising an engaging portion. The frame may include a rear leg comprising a receiving portion disposed to receive the engaging portion. The exercise apparatus may include a moveable backrest. The moveable backrest may include a resilient spine member. The moveable backrest may include a first end connected to the frame. The moveable backrest may include a second end that is freely moveable. The backrest may be disposed to provide support to the user's back during movement of the backrest.