Abstract: A method and system for segmenting tubular or stroke-like structures in 2D images is disclosed. Examples of such structures include, but are not limited to, blood vessels, bones, roads, rivers, electrical wirings, and brush-strokes. User inputs identifying a first region on the image inside of a tubular structure and a second region of the image outside of the tubular structure are received. Based on this information, an ordered series of pearls are generated along the tubular structure. Pearls are 2D disks, each having a center location and a radius determined based on local pixel intensities in the image. A continuous model of the tubular structure is generated by interpolating the center locations and radii of the ordered series of pearls.