Abstract: The present disclosure relates to method for enhanced production of metabolite including but not limiting to isoprene and isoprenoid through chromosomal integration of genes belonging to MEP pathway. The disclosure further relates a host cell for the production of the said metabolite. The method of the present disclosure bypasses the cumbersome method of plasmid application for the production of metabolite. The disclosure also relates to a gene construct comprising MEP genes and auxotrophic markers and a vector comprising the said gene construct.

Abstract: The production of butanol by the mutant Clostridium acetobutylicum MTCC 5587 using an acid pretreated biomass such as jatropha seed cake, pongamia seed cake and banana stems as renewable feedstock. Chemical mutagenesis was carried out for improvement of the strain for better butanol tolerance and production. This leads to a high yield of butanol obtained in single batch fermentation using acid pretreated jatropha seed cake.

Abstract: The present invention provides the use of acid pretreated biomass such as jatropha seed cake, pongamia seed cake and banana stems as renewable feedstock for butanol production by the mutant Clostridium acetobutylicum MTCC 587. Chemical mutagenesis was carried out for improvement of the strain for better butanol tolerance and production. This present invention for the first time has reported an efficient process for high yield of butanol obtained in single batch fermentation using acid pretreated jatropha seed cake.