Abstract: An apparatus comprising a microfluidic device comprising a microfluidic space configured to contain a plurality of microdroplets; a first light source for illuminating the microfluidic device; a voltage source for supplying a voltage to the microfluidic device in order to generate an electric field across the microfluidic space; a first modulator configured to generate a waveform signal to modulate the electric field applied across the microfluidic space, wherein the first modulator is configured to switch between an active state, in which the electric field is applied across the microfluidic space to hold the plurality of microdroplets; and a non-active state, in which the electric field is not applied across the microfluidic space; and an optical imaging device for generating an image of at least a subset of the microdroplets; wherein the first modulator is further configured to, directly or indirectly, control the first light source such that at least a portion of the light is provided across the microflui

Abstract: The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules.

Abstract: The present invention relates to a process for extraction and freeze-drying of a Berberis extract. More particularly, the invention provides an optimized process for freeze-drying/lyophilization to increase the solubility of the extract into the surfactants for enhanced incorporation into a nano-carrier system. Furthermore, the present invention also relates to a lipid nano-formulation of freeze-dried Berberis extract and process for preparing the same by high-pressure homogenization. The invention also relates to the lipid nano-formulation of freeze-dried Berberis extract for efficient drug delivery, for dermatological and cosmeceutical purposes, for treating diabetes and diabetes associated complications, as a wound healer, as an antimicrobial, as an anti-infective, as an anti-inflammatory agent and as an antioxidant.

Abstract: A device for manipulating many hundreds or thousands of microdroplets into an array using EWOD or oEWOD is provided. The device comprises: i) a chip comprising a first region for receiving and manipulating microdroplets; a second region comprising the array and a plurality of electrowetting pathways leading to the array; ii) a microdroplet source configured to provide microdroplets of a predetermined target diameter; iii) a channel configured to provide fluid communication between the microdroplet source and the first region of the chip; and iv) a pressure source configured to move the microdroplets between the microdroplet source and the first region of the chip. The electrowetting pathways on the chip are centre to centre separated by at least double the predetermined target diameter of the microdroplets from the microdroplet source. Furthermore, the controller is configured to enable synchronous movement of the microdroplets in the electrowetting pathways by application of EWOD or oEWOD force.

Abstract: The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules, such as opioids and opioid derivatives.

Abstract: The present invention provides methods and apparatus for manipulating and interrogating the contents of large numbers of microdroplets in parallel on a surface of a microfluidic chip. According to one aspect of the invention a method is provided for manipulating and inspecting microdroplets on a microfluidic chip by optically- mediated electrowetting (oEWOD), the method comprising forming, using a first optical assembly, a plurality of oEWOD traps on a surface of the chip and forming, using a second optical assembly, a second array of oEWOD traps on the surface of the chip, and making an adjustment to the first optical assembly whilst one or more of the microdroplets are held in place by second array of oEWOD traps. Apparatus comprising a microfluidic chip and first and second optical assemblies is also provided.

Abstract: A device for manipulating microdroplets comprises a microfluidic chip adapted to receive and manipulate microdroplets dispersed in a carrier fluid flowing along pathways therethrough characterised in that chip includes regions of differing or zero microdroplet fluid flow rates. Also disclosed is an electrowetting means of transporting emulsions and components of emulsions between the different flow regions.

Abstract: The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules, such as opioids and opioid derivatives.

Abstract: The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules, such as opioids and opioid metabolites.

Abstract: Provided are methods, uses, pharmaceutical regimens, pharmaceutical compositions, and kits comprising modulators of TLR8 and pharmaceutically acceptable salts thereof, useful in treating HIV infections.

Abstract: The present invention encompasses methods of preparing non-segmented negative-stranded RNA viruses from cells utilizing electroporation.

Abstract: The present invention encompasses methods of preparing non-segmented negative-stranded RNA viruses from cells utilizing electroporation.