Patents by Inventor Jason Betley
Jason Betley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11913067Abstract: A composition for amplifying a polynucleotide is provided that includes a substrate comprising a first region and a second region. A first plurality of capture primers is coupled to the first region of the substrate. A second plurality of capture primers is coupled to the second region of the substrate. The capture primers of the second plurality of capture primers are longer than the capture primers of the first plurality of capture primers. A first plurality of orthogonal capture primers are coupled to the first region of the substrate. A second plurality of orthogonal capture primers are coupled to the second region of the substrate. The orthogonal capture primers of the second plurality of orthogonal capture primers are shorter than the orthogonal capture primers of the first plurality of orthogonal capture primers.Type: GrantFiled: August 27, 2021Date of Patent: February 27, 2024Assignees: Illumina, Inc., Illumina Cambridge LimitedInventors: Jeffrey Fisher, Jason Betley
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Publication number: 20220389500Abstract: A method for purifying nucleotides is provided, that includes preparing a solution comprising (a) 3?-blocked nucleotides, (b) 3?-OH nucleotides, (c) a polishing polymerase, and (d) a template. The polishing polymerase and the template are used to selectively polymerize the 3?-OH nucleotides and thus reduce a concentration in the solution of the 3?-OH nucleotides relative to the 3?-blocked nucleotides.Type: ApplicationFiled: May 19, 2022Publication date: December 8, 2022Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITEDInventors: Kim Schneider, Jason Betley, Oliver Miller, Bradley Drews, Dominic Smith, Rajagopal Panchapakesan, Patrick McCauley, Stephen Mason, Tommaso Moschetti, Michael Chesney, Marta Richardson, Elliot Lawrence, Amanda Jackson, Rosamond Jackson, Erin Imsand, Henry Day
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Publication number: 20220186307Abstract: An image sensor structure includes an image layer having an array of light detectors disposed therein. A device stack is disposed over the image layer. An array of light guides is disposed in the device stack. Each light guide is associated with a light detector. An array of nanowells is disposed over the device stack. Each nanowell is associated with a first light guide of the array of light guides. A first primer set is disposed throughout a first well region of each nanowell. A second primer set is disposed throughout a second well region of each nanowell. The second well region is adjacent the first well region. The first and second primer sets are operable to attach a forward strand cluster of forward polynucleotide strands in the first well region and a reverse strand cluster of reverse polynucleotide strands in the second well region.Type: ApplicationFiled: December 9, 2021Publication date: June 16, 2022Applicants: Illumina, Inc., Illumina Cambridge LimitedInventors: Lewis KRAFT, Craig Lee HETHERINGTON, Craig M. CIESLA, Michael BUREK, Jeffrey FISHER, Jason BETLEY
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Publication number: 20220090187Abstract: A composition for amplifying a polynucleotide is provided that includes a substrate comprising a first region and a second region. A first plurality of capture primers is coupled to the first region of the substrate. A second plurality of capture primers is coupled to the second region of the substrate. The capture primers of the second plurality of capture primers are longer than the capture primers of the first plurality of capture primers. A first plurality of orthogonal capture primers are coupled to the first region of the substrate. A second plurality of orthogonal capture primers are coupled to the second region of the substrate. The orthogonal capture primers of the second plurality of orthogonal capture primers are shorter than the orthogonal capture primers of the first plurality of orthogonal capture primers.Type: ApplicationFiled: August 27, 2021Publication date: March 24, 2022Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITEDInventors: Jeffrey Fisher, Jason Betley
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Patent number: 10370709Abstract: Disclosed herein are compositions and methods for sequencing nucleic acids.Type: GrantFiled: September 12, 2016Date of Patent: August 6, 2019Assignee: Illumina Cambridge LimitedInventors: Roberto Rigatti, Jonathan Mark Boutell, Jason Betley, Niall Anthony Gormley
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Publication number: 20160376649Abstract: Disclosed herein are compositions and methods for sequencing nucleic acids.Type: ApplicationFiled: September 12, 2016Publication date: December 29, 2016Applicant: Illumina Cambridge LimitedInventors: Roberto Rigatti, Jonathan Mark Boutell, Jason Betley, Niall Anthony Gormley
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Patent number: 8327724Abstract: Apparatus for preparing a biological sample for PCR analysis has an inlet (4) for receiving the sample (15) and a sieve (13) through which the sample is forced by screwing down an inlet cap (16). A lysis solution between two rupturable seals (11 and 12) is effective to disrupt the cells of the sample and release nucleic acid. The apparatus is mounted releasably on a PCR machine (2) having a motor (2?) releasably coupled with a drive mechanism (3, 36,40) in the apparatus to effect a rotary and a vertical up and down movement of its components (30, 31, 32, 42). The apparatus has a transparent cuvette (5) and a needle (71) extending to the lower end of the cuvette, by which the prepared sample is dispensed to the cuvette for PCR analysis.Type: GrantFiled: February 28, 2008Date of Patent: December 11, 2012Assignee: Smiths Detection-Watford LimitedInventors: Michael Roy Fairs, Thomas David Ford, Piers Sebastian Harding, Gary Stephen Howard, Barry Boyes, John Walter Czajka, Douglas Jason Green, Jay Lewington, Carmelo Volpe, Colin Fewster, Jason Betley, Catherine Mills, William Richard Mawer
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Publication number: 20100261179Abstract: The application provides sample preparation devices and analyses. The devices and analyzes allow for the rapid preparation and analysis of samples using a variety of techniques, including PCR, by even unskilled users.Type: ApplicationFiled: October 17, 2008Publication date: October 14, 2010Inventors: Jason Betley, Barry Boyes, John Walter Czajka, Michael Roy Fairs, Colin Fewster, Thomas David Ford, Douglas Jason Green, Piers Sebastian Harding, Gary Stephen Howard, Jay Lewington, William Richard Mawer, Catherine Mills, James Onslow, Carmelo Volpe
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Publication number: 20100192706Abstract: Apparatus for preparing a biological sample for PCR analysis has an inlet (4) for receiving the sample (15) and a sieve (13) through which the sample is forced by screwing down an inlet cap (16). A lysis solution between two rupturable seals (11 and 12) is effective to disrupt the cells of the sample and release nucleic acid. The apparatus is mounted releasably on a PCR machine (2) having a motor (2?) releasably coupled with a drive mechanism (3, 36,40) in the apparatus to effect a rotary and a vertical up and down movement of its components (30, 31, 32, 42). The apparatus has a transparent cuvette (5) and a needle (71) extending to the lower end of the cuvette, by which the prepared sample is dispensed to the cuvette for PCR analysis.Type: ApplicationFiled: February 28, 2008Publication date: August 5, 2010Inventors: Michael Roy Fairs, Thomas David Ford, Piers Sebastian Harding, Gary Stephen Howard, Barry Boyes, John Walter Czajka, Douglas Jason Green, Jay Lewington, Carmelo Volpe, Colin Fewster, Jason Betley, Catherine Mills, William Richard Mawer
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Publication number: 20080076913Abstract: The invention provides methods for synthesizing oligonucleotides using nucleoside monomers having carbonate protected hydroxyl groups that are deprotected with ?-effect nucleophiles. The ?-effect nucleophile irreversibly cleave the carbonate protecting groups while simultaneously oxidizing the internucleotide phosphite triester linkage to a phosphodiester linkage. The procedure may be carried out in aqueous solution at neutral to mildly basic pH. The method eliminates the need for separate deprotection and oxidation steps, and, since the use of acid to remove protecting groups is unnecessary, acid-induced depurination is avoided. Fluorescent or other readily detectable carbonate protecting groups can be used, enabling monitoring of individual reaction steps during oligonucleotide synthesis. The invention is particularly useful in the highly parallel, microscale synthesis of oligonucleotides.Type: ApplicationFiled: September 6, 2007Publication date: March 27, 2008Inventors: Douglas Dellinger, Marvin Caruthers, Jason Betley
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Publication number: 20060252924Abstract: The invention provides methods for synthesizing oligonucleotides using nucleoside monomers having carbonate protected hydroxyl groups that are deprotected with ?-effect nucleophiles. The ?-effect nucleophile irreversibly cleave the carbonate protecting groups while simultaneously oxidizing the internucleotide phosphite triester linkage to a phosphodiester linkage. The procedure may be carried out in aqueous solution at neutral to mildly basic pH. The method eliminates the need for separate deprotection and oxidation steps, and, since the use of acid to remove protecting groups is unnecessary, acid-induced depurination is avoided. Fluorescent or other readily detectable carbonate protecting groups can be used, enabling monitoring of individual reaction steps during oligonucleotide synthesis. The invention is particularly useful in the highly parallel, microscale synthesis of oligonucleotides.Type: ApplicationFiled: June 15, 2006Publication date: November 9, 2006Inventors: Douglas Dellinger, Marvin Caruthers, Jason Betley
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Publication number: 20060241048Abstract: The invention provides a modified therapeutic agent, said modified agent comprising three or more membrane binding elements with low membrane affinity covalently associated with the agent which elements are capable of interacting independently and with thermodynamic additivity, with components of cellular or artificial membranes exposed to extracellular fluids wherein at least two membrane binding elements are lipophilic elements, which may be aliphatic acyl groups, which may be selected from the list consisting of Myristoyl, Decanoyl or Hexanoyl.Type: ApplicationFiled: September 5, 2003Publication date: October 26, 2006Inventors: Dirk Esser, Jason Betley, Simon Ridley
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Publication number: 20050096456Abstract: This invention relates to compounds, and in particular to polypeptides, their use and methods for their production. The polypeptides are produced from “pseudo-cystein” amino acids that facilitate the assembly of polypeptides by native chemical ligation. The invention provides a compound comprising a polypeptide, the polypeptide having at a C-terminal end a pseudo amino acid, the pseudo amino acid having a side chain containing a 1-amino-2-thiol moiety. For example the polypeptide comprises the structure: formula (I) wherein: R is a polypeptide chain; X is a linker.Type: ApplicationFiled: March 25, 2003Publication date: May 5, 2005Inventor: Jason Betley
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Publication number: 20050090651Abstract: The invention provides a process for forming a compound having the formula (I): from chemical entities native [A], native [B] and native [C], where native [A] has a thioester group, native [B] had a 1-amino-2-thiol group with an unoxidised sulfhydryl side chain, and native [C] had a thiol reactive function (TRF) group, wherein chemical entities [A], [B] and [C] are covalently linked by linker group L, comprising the steps of: (i) admixing native [A] and native [B] in a reaction solution; (ii) condensing the unoxidised sulfydryl side chain of native [B] with the thioester group of native [A] for producing a first intermediate compound wherein [A] and [B] are linked with a ?-aminothioester bond; (iii) rearranging the ?-aminothioester bond for producing a second intermediate compound wherein [A] and [B] are linked with an amide bond having attached thereto a free thiol group; (iv) admixing native [C] with the second intermediate compound in a reaction solution; and (v) reacting the thiol reactive function (TRF)Type: ApplicationFiled: September 26, 2002Publication date: April 28, 2005Applicant: AdProTech Limited Cesterford Research Park, Little Chesterford SaffrInventors: Richard Smith, Jason Betley, Dirk Esser