Abstract: The present invention provides a dialysis-free process for the generation of aqueous regenerated silk fibroin solutions. A degumming reactor is presented that enables scalable batch degumming. As well, the use of diafiltration and desalting columns are introduced for the purification of silk fibroin solutions, representing a set of techniques that isolate solubilized silk fibroin through the efficient removal of a solubilization agent while implicitly availing the increase in concentration of otherwise dilute aqueous silk fibroin solutions.