Abstract: An actuator for a separate downhole tool, including an electric linear actuator, a latch driven by the linear actuator and configured to interact with and operate a movable component of the separate tool. A subsurface safety valve system, including a subsurface safety valve having a flow tube, the valve lacking an operable actuator, and an actuator runnable into the valve, the actuator latchable to the valve to operate the flow tube. A method for controlling a well including installing a subsurface safety valve in a tubing string at a target location in a borehole of the well, the subsurface safety valve lacking an actuator, running an actuator to the valve, engaging the actuator with the valve, and operating the valve with the actuator. A borehole system including a borehole in a subsurface formation, a string in the borehole, and an actuator disposed within or as a part of the string.

Abstract: The subject invention pertains to a method of rapidly transforming a plant with a gene of interest. The method of the current invention comprises the steps of a) preparing a bacterial culture, wherein the bacterial culture comprises a vector containing the gene of interest, b) producing a plant cell suspension culture from the plant, c) contacting the plant cell suspension culture with the bacterial culture to produce a plant cell transformed with the gene of interest from the plant cell suspension culture, and e) producing a plant from the plant cell transformed with the gene of interest. The method of the current invention can be designed for a high throughput transformation and screening of a plant with a plurality of genes of interest and screening the plants to identify and obtain plants having desirable characteristics.