Abstract: A method for operating a chromatography setup of a bioprocess installation with a plurality of chromatography columns, each with a column inlet and a column outlet, and a valve switching cassette with a group of inlet ports, a group of outlet ports, a group of column-in ports and a group of column-out ports. It is prosed, that a first liquid stream of concentrated buffer is introduced into a first internal liquid line via a first inlet port and that a second liquid stream of diluent is introduced into a second internal liquid line via a second inlet port, that in a dilution process, the array of valve units is switched as to create a third liquid stream by merging the first liquid stream and the second liquid stream at a merging location within the valve switching cassette.

Abstract: A method for operating a chromatography setup of a bioprocess installation with a plurality of chromatography columns, each with a column inlet and a column outlet, and a valve switching cassette with a group of inlet ports, a group of outlet ports, a group of column-in ports and a group of column-out ports . It is prosed, that a first liquid stream of concentrated buffer is introduced into a first internal liquid line via a first inlet port and that a second liquid stream of diluent is introduced into a second internal liquid line via a second inlet port, that in a dilution process, the array of valve units is switched as to create a third liquid stream by merging the first liquid stream and the second liquid stream at a merging location within the valve switching cassette.