Abstract: Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.

Abstract: Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.

Abstract: One embodiment of the present invention provides a switch. During operation, the switch maintains a first counter to indicate a first number of packets in a queue of the switch. The switch then determines whether a clock of the switch has reached a threshold value. If the clock reaches the threshold value, the switch starts maintaining a second counter to indicate a second number of packets in the queue that have been received after the clock has reached the threshold value. The switch continues to decrement the first counter in response to a packet leaving the queue until the first counter reaches a value of zero. When the clock reaches a maximum value supported by the clock, the switch drops a third number of packets from the queue indicated by the first counter.

Abstract: One embodiment of the present invention provides a switch. During operation, the switch maintains a first counter to indicate a first number of packets in a queue of the switch. The switch then determines whether a clock of the switch has reached a threshold value. If the clock reaches the threshold value, the switch starts maintaining a second counter to indicate a second number of packets in the queue that have been received after the clock has reached the threshold value. The switch continues to decrement the first counter in response to a packet leaving the queue until the first counter reaches a value of zero. When the clock reaches a maximum value supported by the clock, the switch drops a third number of packets from the queue indicated by the first counter.

Abstract: A rework re-timer with forward error correction handling is disclosed. An example first intermediate transceiver includes a first interface to communicatively couple the first intermediate transceiver with a first computing device, a second interface to communicatively couple the first intermediate transceiver to a second intermediate transceiver configured to be communicatively coupled with a second computing device, an auto-negotiation controller to: terminate a first auto-negotiation with the first computing device before the first auto-negotiation is completed, transmit, to the second transceiver, first capabilities of the first computing device determined during the first auto-negotiation, and perform a second auto-negotiation with the first computing device utilizing the first capabilities of the first computing device and second capabilities of the second computing device received from the second transceiver.

Abstract: Provided are compositions and methods for labeling an endogenous protein, in particular, in a live cell, or for ablating an endogenous or target protein. The compositions relate to a fusion protein having a binding moiety such as an antibody, an antigen binding fragment of an antibody or an antibody mimetic that recognizes the endogenous or target protein.

Abstract: A method of providing directed, two-way consultative communications between a consumer and a customer service representative comprises: establishing a storyboard layout by a company to provide a guideline for the customer service representative to follow; receiving a request by the consumer for a consultation regarding a product or service offered by the company; initiating a two-way online web browser session between the consumer and the customer service representative; pushing content from customer service representative computing device to a consumer computing device, wherein the content is pulled from a server of the company and place in the storyboard layout; and returning data from the consumer computing device to the customer service representative device in response to the content pushed from the customer service representative computing device to the consumer computing device.

Abstract: A network re-timer with forward error correction handling is disclosed. An example network re-timer includes a first receiver to receive data from a first connected device and to re-time the data to generate re-timed data, a first transmitter to transmit the re-timed data to a second connected device, a first auto-negotiation handler communicatively coupled to the first receiver to control a first forward error correction mode for communications with the first connected device, and a second auto-negotiation handler communicatively coupled to the first transmitter to control a second forward error correction mode for communications with the second connected device, wherein the first forward error correction mode is different than the second forward error correction mode.

Abstract: Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.

Abstract: A network re-timer with forward error correction handling is disclosed. An example network re-timer includes a first receiver to receive data from a first connected device and to re-time the data to generate re-timed data, a first transmitter to transmit the re-timed data to a second connected device, a first auto-negotiation handler communicatively coupled to the first receiver to control a first forward error correction mode for communications with the first connected device, and a second auto-negotiation handler communicatively coupled to the first transmitter to control a second forward error correction mode for communications with the second connected device, wherein the first forward error correction mode is different than the second forward error correction mode.

Abstract: A rework re-timer with forward error correction handling is disclosed. An example first intermediate transceiver includes a first interface to communicatively couple the first intermediate transceiver with a first computing device, a second interface to communicatively couple the first intermediate transceiver to a second intermediate transceiver configured to be communicatively coupled with a second computing device, an auto-negotiation controller to: terminate a first auto-negotiation with the first computing device before the first auto-negotiation is completed, transmit, to the second transceiver, first capabilities of the first computing device determined during the first auto-negotiation, and perform a second auto-negotiation with the first computing device utilizing the first capabilities of the first computing device and second capabilities of the second computing device received from the second transceiver.

Abstract: Provided are compositions and methods for labeling an endogenous protein, in particular, in a live cell, or for ablating an endogenous or target protein. The compositions relate to a fusion protein having a binding moiety such as an antibody, an antigen binding fragment of an antibody or an antibody mimetic that recognizes the endogenous or target protein.

Abstract: A method of providing directed, two-way consultative communications between a consumer and a customer service representative comprises: establishing a storyboard layout by a company to provide a guideline for the customer service representative to follow; receiving a request by the consumer for a consultation regarding a product or service offered by the company; initiating a two-way online web browser session between the consumer and the customer service representative; pushing content from customer service representative computing device to a consumer computing device, wherein the content is pulled from a server of the company and place in the storyboard layout; and returning data from the consumer computing device to the customer service representative device in response to the content pushed from the customer service representative computing device to the consumer computing device.

Abstract: Provided are compositions and methods for labeling an endogenous protein, in particular, in a live cell, or for ablating an endogenous or target protein. The compositions relate to a fusion protein having a binding moiety such as an antibody, an antigen binding fragment of an antibody or an antibody mimetic that recognizes the endogenous or target protein.

Abstract: A storage container with retractable stands has a body and two stand assemblies. The body has a front surface, a rear surface and multiple slots. The slots are formed in the front and rear surfaces. Each stand assembly has multiple props. Each prop is mounted pivotally in a corresponding slot. The props are pivoted to stand on the ground to stand the storage container off the ground for increased convenience. When the props are held in the slots, the props are completely received in the slots to avoid additional storage volume.

Abstract: A storage container with retractable stands has a body and two stand assemblies. The body has a front surface, a rear surface and multiple slots. The slots are formed in the front and rear surfaces. Each stand assembly has multiple props. Each prop is mounted pivotally in a corresponding slot. The props are pivoted to stand on the ground to stand the storage container off the ground for increased convenience. When the props are held in the slots, the props are completely received in the slots to avoid additional storage volume.

Abstract: A foldable storage container can be used as a suitcase or to hold and transport sports balls and has a rear panel, two side panels, a base, a front panel and a cover. The rear panel has a top, a bottom and two side edges. The side panels are respectively formed perpendicularly on the side edges of the rear panel and are foldable. The base is mounted pivotally on the rear panel. The front panel is mounted slidably between the side panels and has a top. The cover is mounted pivotally and detachably on the top of the rear panel and is selectively locked on the top of the front panel. Therefore, the foldable storage container can be folded to occupy a smaller space for convenient storage.