Abstract: In various embodiments, methods for producing aqueous polyisoprene latex from natural cis-1,4-polyisoprene are described. The natural cis-1,4-polyisoprene may be sourced from guayule scrubs. In various embodiments, the method comprises extracting guayule plant material to form a miscella, fractionating the miscella to a preliminary cement, diluting the preliminary cement to a cement for dispersing, dispersing the cement in an aqueous surfactant mixture under high shear to produce an emulsion, and de-solventizing the emulsion to produce an aqueous latex dispersion. The rubber solids level can then be adjusted by centrifugation and dilution in water to produce a final aqueous cis-1,4-polyisoprene latex.