Patents by Inventor Jason Komadina
Jason Komadina has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11739380Abstract: A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other.Type: GrantFiled: March 12, 2021Date of Patent: August 29, 2023Assignee: Roche Sequencing Solutions, Inc.Inventors: Geoffrey Barrall, Jason Komadina, Marcin Rojek
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Publication number: 20230087757Abstract: A nanopore-based sequencing chip can have a surface with an array of wells, with each well having a working electrode. Charge can be established within the wells by applying a voltage between the working electrodes and a counter electrode. The charge can then be trapped within the wells by sealing the wells with a membrane. The trapped charge can be used to facilitate pore insertion into the membranes.Type: ApplicationFiled: November 1, 2022Publication date: March 23, 2023Inventors: Geoffrey BARRALL, George John CARMAN, Harikrishnan JAYAMOHAN, Jason KOMADINA, J. William MANEY, JR.
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Publication number: 20220042968Abstract: Systems and methods for inserting a nanopore into a membrane covering a well are described herein. The membrane can be bowed outwards by establishing an osmotic gradient across the membrane in order to drive fluid into the well, which will increase the amount of fluid in the well and cause the membrane to bow outwards. Nanopore insertion can then be initiated on the bowed membrane.Type: ApplicationFiled: October 22, 2021Publication date: February 10, 2022Inventors: Geoffrey Barrall, Ashwini Bhat, Michael Dorwart, Jason Komadina, George Carman, Hannah Kallewaard-Lum, Kyle Umeda, Wooseok Jung, Yufang Wang
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Publication number: 20210381046Abstract: A nanopore cell includes a conductive layer. The nanopore cell further includes a titanium nitride (TiN) working electrode disposed above the conductive layer. The nanopore cell further includes insulating walls disposed above the TiN working electrode, wherein the insulating walls and the TiN working electrode form a well into which an electrolyte may be contained. In some embodiments, the TiN working electrode comprises a spongy and porous TiN working electrode that is deposited by a deposition technique with conditions tuned to deposit sparsely-spaced TiN columnar structures or columns of TiN crystals above the conductive layer.Type: ApplicationFiled: August 23, 2021Publication date: December 9, 2021Inventors: John Foster, Jason Komadina
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Publication number: 20210311017Abstract: Disclosed herein are ruthenium-containing materials, such as ruthenium containing materials having a double layer capacitance ranging from between about 180 pF/um2 to about 320 pF/um2. In some embodiments, the ruthenium-containing materials are suitable for use in electrodes. In some embodiments, the ruthenium-containing materials are suitable for use in nanopore sequencing devices.Type: ApplicationFiled: February 25, 2021Publication date: October 7, 2021Inventors: Wing Kei Au, Jason Komadina, Marowen Ng
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Publication number: 20210302409Abstract: Systems and methods for inserting a single pore into a membrane are described herein. A stepped or ramped voltage waveform can be applied across the membranes of the cells of an array, where the voltage waveform starts at first voltage and increases in magnitude over a period of time to a second voltage. The first voltage is selected to be low enough to reduce the risk of damaging the membrane, while the rate of voltage increase is selected to provide sufficient time for the pores to insert into the membranes. Once a pore is inserted into the membrane, the voltage across the membrane rapidly drops, thereby reducing the risk of damaging the membrane even if the applied voltage between the electrodes is further increased.Type: ApplicationFiled: June 10, 2021Publication date: September 30, 2021Inventors: Geoffrey Barrall, George John Carman, Takeshi Harada, Jason Komadina, J. William Maney, JR., Charlotte Yang
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Patent number: 11098354Abstract: A nanopore cell includes a conductive layer. The nanopore cell further includes a titanium nitride (TiN) working electrode disposed above the conductive layer. The nanopore cell further includes insulating walls disposed above the TiN working electrode, wherein the insulating walls and the TiN working electrode form a well into which an electrolyte may be contained. In some embodiments, the TiN working electrode comprises a spongy and porous TiN working electrode that is deposited by a deposition technique with conditions tuned to deposit sparsely-spaced TiN columnar structures or columns of TiN crystals above the conductive layer.Type: GrantFiled: November 27, 2018Date of Patent: August 24, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: John Foster, Jason Komadina
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Publication number: 20210198736Abstract: A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other.Type: ApplicationFiled: March 12, 2021Publication date: July 1, 2021Inventors: Geoffrey BARRALL, Jason KOMADINA, Marcin ROJEK
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Patent number: 10947590Abstract: A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other.Type: GrantFiled: January 29, 2020Date of Patent: March 16, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: Geoffrey Barrall, Jason Komadina, Marcin Rojek
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Publication number: 20200246791Abstract: Techniques for replacing nanopores within a nanopore based sequencing chip are provided. A first electrolyte solution is added to the external reservoir of the sequencing chip, introducing an osmotic imbalance between the reservoir and the well chamber located on the opposite side of a lipid bilayer membrane. The osmotic imbalance causes the membrane to change shape, and a nanopore within the membrane to be ejected. A second electrolyte solution is then added to the external reservoir to provide replacement nanopores and to restore the membrane shape. The replacement nanopores can be inserted into the membrane, effectively replacing the initial pore without causing the destruction of the membrane.Type: ApplicationFiled: April 20, 2020Publication date: August 6, 2020Inventors: Geoffrey Barrall, Takeshi Harada, Jason Komadina, Pirooz Parvarandeh, Charlotte Yang
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Publication number: 20200165670Abstract: A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other.Type: ApplicationFiled: January 29, 2020Publication date: May 28, 2020Inventors: Geoffrey BARRALL, Jason KOMADINA, Marcin ROJEK
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Patent number: 10577653Abstract: A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other.Type: GrantFiled: June 22, 2017Date of Patent: March 3, 2020Assignee: Roche Sequencing Solutions, Inc.Inventors: Geoffrey Barrall, Jason Komadina, Marcin Rojek
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Patent number: 10371664Abstract: A nanopore cell includes a titanium nitride (TiN) counter electrode configured to be at a first electric potential. The nanopore cell also include a working electrode configured to be at a second electric potential and an insulating wall. The insulating wall and the working electrode form at least a portion of a well configured to contain an electrolyte at a voltage that is at least a portion of an electric potential difference between the first electric potential of the titanium nitride counter electrode and the second electric potential of the working electrode.Type: GrantFiled: January 19, 2017Date of Patent: August 6, 2019Assignee: Roche Molecular Systems, Inc.Inventors: Markus Wallgren, Janusz B. Wójtowicz, Robert A. Yuan, John Foster, Jason Komadina
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Publication number: 20190161795Abstract: A nanopore cell includes a conductive layer. The nanopore cell further includes a titanium nitride (TiN) working electrode disposed above the conductive layer. The nanopore cell further includes insulating walls disposed above the TiN working electrode, wherein the insulating walls and the TiN working electrode form a well into which an electrolyte may be contained. In some embodiments, the TiN working electrode comprises a spongy and porous TiN working electrode that is deposited by a deposition technique with conditions tuned to deposit sparsely-spaced TiN columnar structures or columns of TiN crystals above the conductive layer.Type: ApplicationFiled: November 27, 2018Publication date: May 30, 2019Inventors: John Foster, Jason Komadina
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Patent number: 10174371Abstract: A nanopore cell includes a conductive layer. The nanopore cell further includes a titanium nitride (TiN) working electrode disposed above the conductive layer. The nanopore cell further includes insulating walls disposed above the TiN working electrode, wherein the insulating walls and the TiN working electrode form a well into which an electrolyte may be contained. In some embodiments, the TiN working electrode comprises a spongy and porous TiN working electrode that is deposited by a deposition technique with conditions tuned to deposit sparsely-spaced TiN columnar structures or columns of TiN crystals above the conductive layer.Type: GrantFiled: August 5, 2015Date of Patent: January 8, 2019Assignee: Genia Technologies, Inc.Inventors: John Foster, Jason Komadina
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Publication number: 20170369944Abstract: A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other.Type: ApplicationFiled: June 22, 2017Publication date: December 28, 2017Inventors: Geoffrey BARRALL, Jason KOMADINA, Marcin ROJEK
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Publication number: 20170212079Abstract: A nanopore cell includes a titanium nitride (TiN) counter electrode configured to be at a first electric potential. The nanopore cell also include a working electrode configured to be at a second electric potential and an insulating wall. The insulating wall and the working electrode form at least a portion of a well configured to contain an electrolyte at a voltage that is at least a portion of an electric potential difference between the first electric potential of the titanium nitride counter electrode and the second electric potential of the working electrode.Type: ApplicationFiled: January 19, 2017Publication date: July 27, 2017Inventors: Markus Wallgren, Janusz B. Wójtowicz, Robert A. Yuan, John Foster, Jason Komadina
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Publication number: 20170037467Abstract: A nanopore cell includes a conductive layer. The nanopore cell further includes a titanium nitride (TiN) working electrode disposed above the conductive layer. The nanopore cell further includes insulating walls disposed above the TiN working electrode, wherein the insulating walls and the TiN working electrode form a well into which an electrolyte may be contained. In some embodiments, the TiN working electrode comprises a spongy and porous TiN working electrode that is deposited by a deposition technique with conditions tuned to deposit sparsely-spaced TiN columnar structures or columns of TiN crystals above the conductive layer.Type: ApplicationFiled: August 5, 2015Publication date: February 9, 2017Inventors: John Foster, Jason Komadina