Abstract: The present disclosure provides isolated and synthetic DNA and cDNA molecules encoding a phytase from the root of Pteris vittata (PV); root PV phytase proteins and peptides; root PV phytase antisense molecules, vectors, transgenic cells and plants containing root PV phytase nucleic acid molecules, isolated polypeptides, or antisense molecules; genetic markers for root PV phytase; and methods of using these nucleic acid or polypeptide molecules to improve phosphorus utilization from phytate by plants and animals.