Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

Abstract: An imaging compatible device for applying a plurality of different loads to a foot for evaluating the Lisfranc joint of a patient is configured to move the ball of the foot in an axial direction, a lateral direction, and a torsional direction to assess a state of the Lisfranc joint.

Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.

Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.

Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents. Further disclosed are nucleic acid compositions with reduced cytotoxicity.

Abstract: The following invention relates to smart material couplings, particularly to shape memory alloy suspension systems to mitigate against shock or blast. There is provided A land vehicle comprising: —an armoured v shaped hull; at least one wheel set with a hub, and at least one suspension device comprising a shape memory material operably connecting the hull to the wheel set.

Abstract: An imaging compatible device for applying a plurality of different loads to a foot for evaluating the Lisfranc joint of a patient is configured to move the ball of the foot in an axial direction, a lateral direction, and a torsional direction to assess a state of the Lisfranc joint.

Abstract: An anvil adjustment assembly of a vertical shaft impactor includes an anvil ring configured to be disposed within a housing of the vertical shaft impactor. The anvil ring includes a tab which is coupled to an elongate jack bolt having a first end and a second end. A jack bolt housing partially surrounds the bolt and is coupled to the housing. A first locking collar and a second locking collar are coupled to the jack bolt. The first locking nut is coupled to the jack bolt on one side of the tab, and the second locking nut is coupled to the jack bolt on the opposite side of the tab. The jack bolt moves along a longitudinal axis within the jack bolt housing between a first position and a second position, and the movement of the jack bolt along the longitudinal axis induces longitudinal movement of the anvil ring.

Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

Abstract: The following invention relates to smart material couplings, particularly to shape memory alloy drivetrain systems to mitigate against shock or blast. There is provided an armoured land vehicle comprising:—an armoured v shaped hull; a powerplant located within said chassis, at least one wheel set with a hub, and, at least one drive shaft comprising a shape memory alloy, wherein said drivetrain is located between and operably connected via drive couplings to said powerplant and the hub of the at least one wheel set, to provide drive to said at least one wheel set.

Abstract: The following invention relates to split hulls, particularly to V shaped split hulls with a biased resilient member to mitigate against shock or blast. There is provided an armoured land vehicle comprising; a lower armoured v shaped hull; an upper hull which is located above and slidably engaged in a vertical plane with said hull, wherein there is at least one biased resilient member located between said upper hull and lower hull, to reduce the travel between said upper and lower hulls in the event of a shock event.

Abstract: The present disclosure generally relates to compositions and methods for genetic modification of cells. In particular, the disclosure relates to stabilized reagents for genome alteration (e.g., site specific nucleases), as well as stabilized reagents for non-genetic code altering modification (e.g., DNA methylation). Stabilization methods include storage of reagents at suitable temperatures, conversion to dry forms, and chemical modifications.

Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

Abstract: The invention provides novel reverse transcriptases (RTs) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.

Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.

Abstract: A computing device (e.g., a server computer hosted by a vehicle manufacturer or retailer) receives vehicle configuration information obtained via a user interface of a user device. The vehicle configuration information includes one or more user-adjustable settings. The computing device determines whether a conflict exists between the user-adjustable settings and a regulated vehicle parameter (e.g., a vehicle speed limit), which may be associated with greenhouse gas emissions of the vehicle. The computing device transmits feedback information to the user device. The feedback information may include a description of one or more conflicts between the user-adjustable settings and the regulated vehicle parameter, recommended adjustments to the settings, or the like. The recommended adjustments to the settings may, if accepted by a user, improve regulatory compliance for the vehicle, and may remove or reduce the degree of conflicts between the user-adjustable setting and the regulated vehicle parameter.

Abstract: An isolated mutant Tfi DNA polymerase having a D144A point mutation. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis.

Abstract: A computing device (e.g., a server computer hosted by a vehicle manufacturer or retailer) receives vehicle configuration information obtained via a user interface of a user device. The vehicle configuration information includes one or more user-adjustable settings. The computing device determines whether a conflict exists between the user-adjustable settings and a regulated vehicle parameter (e.g., a vehicle speed limit), which may be associated with greenhouse gas emissions of the vehicle. The computing device transmits feedback information to the user device. The feedback information may include a description of one or more conflicts between the user-adjustable settings and the regulated vehicle parameter, recommended adjustments to the settings, or the like. The recommended adjustments to the settings may, if accepted by a user, improve regulatory compliance for the vehicle, and may remove or reduce the degree of conflicts between the user-adjustable setting and the regulated vehicle parameter.