Abstract: A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagent droplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.

Abstract: A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagent droplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.

Abstract: A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagent droplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.

Abstract: A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagent droplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.

Abstract: A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagent droplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.

Abstract: A method of droplet manipulation utilizing a droplet manipulation device includes activating elements of the device to bring a first droplet into proximity of a second droplet, controlling the elements of the device to alter the shape of at least one of the first and second droplets, and further controlling the elements of the device to move at least one of the first or second droplets until the droplets are in contact about an aggregate area. The elements are controlled in a manner so as to control the area of contact and the degree of mixing of the fluid between the first and second droplets. The method may be employed to move particles of a particulate suspension from the first droplet to the second droplet. The droplet manipulation device may be an electrowetting on dielectric (EWOD) device, which includes shaping electrodes activated to shape droplets, and a bridging electrode activated to join the droplets to transfer fluid between the shaped droplets.

Abstract: An active matrix device is provided which includes N array elements arranged spatially in a sequence of first through Nth array elements (where N is an integer ?2); the N array elements each including a write input for receiving a corresponding write input signal which controls operation of the array element, and a sense circuit for sensing a property of the array element and providing a sensor output based on the sensed property; and further including a manipulation circuit including logic circuitry connecting the sensor output from an nth array element in the sequence directly to the write input of an (n+1)th array element and configured to provide the write input signal to the write input of the (n+1)th array element based on the sensor output from the nth array element.

Abstract: A method of droplet manipulation utilizing a droplet manipulation device includes activating elements of the device to bring a first droplet into proximity of a second droplet, controlling the elements of the device to alter the shape of at least one of the first and second droplets, and further controlling the elements of the device to move at least one of the first or second droplets until the droplets are in contact about an aggregate area. The elements are controlled in a manner so as to control the area of contact and the degree of mixing of the fluid between the first and second droplets. The method may be employed to move particles of a particulate suspension from the first droplet to the second droplet.

Abstract: A microfluidic system includes a microfluidic device; and a metered fluid loading system formed integrally with the microfluidic device and configured to load a discrete metered volume of fluid into the microfluidic device upon actuation.

Abstract: An array element for a temperature sensor array circuit. The array element includes a switch transistor; and a temperature sensor element having an impedance which varies as a function of temperature, the temperature sensor element being connected in parallel with a source and drain of the switch transistor.

Abstract: An active matrix device is provided which includes N array elements arranged spatially in a sequence of first through Nth array elements (where N is an integer ?2); the N array elements each including a write input for receiving a corresponding write input signal which controls operation of the array element, and a sense circuit for sensing a property of the array element and providing a sensor output based on the sensed property; and further including a manipulation circuit including logic circuitry connecting the sensor output from an nth array element in the sequence directly to the write input of an (n+1)th array element and configured to provide the write input signal to the write input of the (n+1)th array element based on the sensor output from the nth array element.

Abstract: An active matrix electrowetting on dielectric (AM-EWOD) device which includes a plurality of array elements configured to manipulate one or more droplets of fluid on an array, each of the array elements including a corresponding array element circuit. Each array element circuit includes a top substrate electrode and a drive electrode between which the one or more droplets may be positioned; circuitry configured to write data to the corresponding array element by selectively applying to the drive electrode either: (i) a time-varying voltage waveform V1 of amplitude VB and period t0; or (ii) a time-varying voltage waveform V2, the logical inverse of V1, and applying to the top substrate electrode the time-varying voltage waveform V2+Voffset, where Voffset represents an offset voltage signal which may have AC and/or DC components and may equal zero.

Abstract: An array element for a temperature sensor array circuit.

Abstract: Physical barriers (210) are present between neighbouring pixels (200) on a circuit substrate (100) of an active-matrix electroluminescent display device, particularly with LEDs (25) of organic semiconductor materials. The invention forms these barriers (210) with metal or other electrically-conductive material (240) that serves as an interconnection between a first circuit element (21, 4, 5, 6, 140, 150, 160, T1, T2, Tm, Tg, Ch) of the circuit substrate and a second circuit element (400, 400s, 23), for example, a sensor (400s) of a sensor array supported over the pixel array. The conductive barrier material (240) is insulated (40) at the sides of the barriers adjacent to the LEDs and has an un-insulated top connection area (240t) at which the second circuit element is connected to the conductive barrier material (240).

Abstract: Physical barriers (210) are present between neighboring pixels (200) on a circuit substrate (100) of an active-matrix display device, such as an electroluminescent display formed with LEDs (25) of organic semiconductor materials. The invention forms at least parts of the barriers (210) with metal or other electrically-conductive material (240) that is insulated (40) from the LEDs but connected to the circuitry (4, 5, 6, 9, 140, 150, 160, T1, T2, Tm, Tg, Ch etc.) within the substrate (100). This conductive barrier material (240) may back up or replace, for example, matrix addressing lines (150) and/or form an additional component either within the pixel array or outside. The additional component comprising the conductive barrier material (240) is advantageously a capacitor (Ch), or an inductor (L) or transformer (W), or even an aerial.

Abstract: Physical barriers (210) are present between neighbouring pixels (200) on a circuit substrate (100) of an active-matrix electroluminescent display device, particularly with LEDs (25) of organic semiconductor materials. In order to reduce parasitic capacitance in the circuit substrate, the invention forms these barriers (210) with metal or other electrically conductive material (240) that provides at least part of the signal lines (160) at a higher level than the circuit substrate (100). This conductive barrier material (240) is connected to the matrix circuitry within the substrate (100) but is insulated (40) at least at the sides adjacent to the LEDs (25). Preferably, an inter-capacitance guard line (9) is included in the circuit substrate (100) between the signal lines (160) and the circuitry in the substrate (100).

Abstract: Physical barriers (210) are present between neighbouring pixels (200) on a circuit substrate (100) of an active-matrix electroluminescent display device, particularly with LEDs (25) of organic semiconductor materials. The invention forms these barriers (210) with metal or other electrically-conductive material (240) that serves as an interconnection between a first circuit element (21, 4, 5, 6, 140, 150, 160, T1, T2, Tm, Tg, Ch) of the circuit substrate and a second circuit element (400, 400s, 23), for example, a sensor (400s) of a sensor array supported over the pixel array. The conductive barrier material (240) is insulated (40) at the sides of the barriers adjacent to the LEDs and has an un-insulated top connection area (240t) at which the second circuit element is connected to the conductive barrier material (240).

Abstract: Physical barriers (210) are present between neighbouring pixels (200) on a circuit substrate (100) of an active-matrix electroluminescent display device, particularly with LEDs (25) of organic semi conductor materials. The invention forms these barriers (210) with metal or other electrically-conductive material (240), that is insulated (40) from the LEDs but connected to the circuitry within the substrate (100). This conductive barrier material (240) backs-up or replaces at least a part of the drive supply line (140,240) to which the LEDs are connected by a drive element T1. This transfers the problem of line resistance and associated voltage drop from within the circuit substrate (100), where it is severely constrained, to the much freer environment of the pixel barriers (210) on the substrate (100) where the conductive barrier material (240) can provide much lower resistance. Very large displays can be made with low voltage drops along this composite drive supply line (140,240).

Abstract: Physical barriers (210) are present between neighbouring pixels (200) on a circuit substrate (100) of an active-matrix electroluminescent display device, particularly with LEDs (25) of organic semiconductor materials. The invention forms these barriers (210) with metal or other electrically-conductive material (240) that serves as an interconnection between a first circuit element (21, 4, 5, 6, 140, 150, 160, T1, T2, Tm, Tg, Ch) of the circuit substrate and a second circuit element (400, 400s, 23), for example, a sensor (400s) of a sensor array supported over the pixel array. The conductive barrier material (240) is insulated (40) at the sides of the barriers adjacent to the LEDs and has an un-insulated top connection area (240t) at which the second circuit element is connected to the conductive barrier material (240).