Abstract: The invention disclosed herein generally relates to methods and systems for growing, expanding and/or obtaining 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity. In particular, the invention disclosed herein relates to methods and systems for growing human cells having SOX9 protein activity and SOX2+ protein activity in vitro, and for promoting pluripotent stem cell derived ventral-anterior foregut spheroid tissue into 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity.

Abstract: The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or cells through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting functional basal-like cells from pluripotent stem cell-derived lung bud tip progenitor organoid tissue through activation of SMAD signaling via activation of TGF?1 (and/or the TGF? signaling pathway) and BMP4 (and/or the BMP signaling pathway).

Abstract: The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or cells through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting functional basal-like cells from pluripotent stem cell-derived lung bud tip progenitor organoid tissue through activation of SMAD signaling via activation of TGF?1 (and/or the TGF? signaling pathway) and BMP4 (and/or the BMP signaling pathway).

Abstract: The invention disclosed herein generally relates to methods and systems for improving physiological growth of cultured tissues. In particular, the invention disclosed herein relates to methods and systems for promoting maintenance of cultured intestinal organoids (e.g., derived from pluripotent stem cells or from primary sources such as biopsy tissue).

Abstract: The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or organ(s) through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting vascularized human intestinal organoid tissue having an intestine-specific EC transcriptional signature from hindgut spheroid tissue produced in vitro from the described methods.

Abstract: The present application is directed to compositions and methods for establishing organoid cultures from cryogenically preserved tissue (e.g., human biopsy tissue), and related methods of use with such established organoid cultures.

Abstract: The present invention relates to a control system for a refuse vehicle and a method for controlling a refuse vehicle provided with at least one refuse container location sensor, at least one vehicle speed control, and one or more electronics. The one or more electronics receive and process signals from the at least one refuse container location sensor and outputs a control signal to the at least one vehicle speed control to stop the refuse vehicle at a predetermined location with respect to a refuse container.

Abstract: The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or cells through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting functional basal-like cells from pluripotent stem cell-derived lung bud tip progenitor organoid tissue through activation of SMAD signaling via activation of TGF?1 (and/or the TGF?signaling pathway) and BMP4 (and/or the BMP signaling pathway).

Abstract: The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or organ(s) through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting definitive endoderm formation from pluripotent stem cells.

Abstract: The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or organ(s) through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting definitive endoderm formation from pluripotent stem cells. The invention disclosed herein further relates to methods and systems for promoting ventral-anterior foregut spheroid tissue formation, 3-dimensional lung tissue formation, and lung organoid tissue formation produced in vitro from the described methods.

Abstract: The invention disclosed herein generally relates to methods and systems for growing, expanding and/or obtaining 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity. In particular, the invention disclosed herein relates to methods and systems for growing human cells having SOX9 protein activity and SOX2+ protein activity in vitro, and for promoting pluripotent stem cell derived ventral-anterior foregut spheroid tissue into 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity.

Abstract: The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or organ(s) through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting definitive endoderm formation from pluripotent stem cells.

Abstract: The present invention relates to a control system for a refuse vehicle and a method for controlling a refuse vehicle provided with at least one refuse container location sensor, at least one vehicle speed control, and one or more electronics. The one or more electronics receive and process signals from the at least one refuse container location sensor and outputs a control signal to the at least one vehicle speed control to stop the refuse vehicle at a predetermined location with respect to a refuse container.

Abstract: A system for automatically detaching a windshield from a vehicle cab comprises a pressure applying device mounted between a windshield and a frame element, a roll-over condition sensor, and a controller connected to receive a signal indicating a roll-over condition from the roller-over condition sensor and connected to activate the pressure applying device responsive to receiving said signal. The pressure applying device may be one or more pyrotechnic devices. The system includes a delay function to delay activating the pressure applying device to allow the vehicle to come to a rest.

Abstract: A system for automatically detaching a windshield from a vehicle cab comprises a pressure applying device mounted between a windshield and a frame element, a roll-over condition sensor, and a controller connected to receive a signal indicating a roll-over condition from the roller-over condition sensor and connected to activate the pressure applying device responsive to receiving said signal. The pressure applying device may be one or more pyrotechnic devices. The system includes a delay function to delay activating the pressure applying device to allow the vehicle to come to a rest.

Abstract: An adjustable roof extension for a vehicle that has a cab portion and a trailer portion includes a roof extension adapted for disposition at a rear of a roof of the cab portion and an adjustment mechanism that attaches the roof extension to the cab portion such that the roof extension is pivotable and that an end of the roof extension that is nearer the trailer portion is controllably movable to pull an air flow over the cab portion toward the trailer portion.

Abstract: In one embodiment, a method is provided for making an array plate for high throughput assays.

Abstract: A heavy duty truck includes a grab handle for installation on the exterior of the truck to aid operator access to the vehicle. The handle includes an inner and outer tubular member. The inner tubular member has two opposing ends, a closed end formed to define a first truck mating surface and an open end. The outer tubular member has two opposing ends, a closed end formed to define a second truck mating surface and an open end. The inner tubular member is slideably engaged within the outer tubular member such that the inner and outer tubular members are coaxial and define a length of the grab handle. The length is adjustable over a range by sliding the inner tubular member with respect to the outer tubular member. The handle may include an annular shaped seal mounted on the outer tubular member open end to protect an operator's hand.