Abstract: A latent effervescent body comprising a selective agent is disclosed. A method of using the latent effervescent body in a method to selectively enrich a target microorganism is also disclosed. The method comprises providing a sample, a culture medium, and the latent effervescent body. The method further comprises contacting the sample, the culture medium, and the latent effervescent body under conditions to facilitate growth of the target microorganism. The method further comprises releasing the selective agent from the latent effervescent body. Optionally, the method includes detecting a microorganism.

Abstract: A tool (100) is provided for use in uncapping a plurality of linearly-oriented, spaced-apart tubes; the tubes capped with a unitary closure device comprising a plurality of spaced-apart, linearly-oriented alternating caps and openings, each cap having a cap upper surface and being connected to at least one adjacent cap by at least two connecting structures, each of the at least two connecting structures having a connecting structure upper surface and a connecting structure lower surface, the at least two connecting structures and two adjacent caps forming a boundary of an opening. The tool comprises a body (30) having first portion (42) for engagement by a user and a second portion comprising a base (52) and a plurality of spaced-apart projections (60) extending there-from. Each projection is configured for releasably engaging one of the openings in the unitary closure device. Optionally, the first portion is configured in a non-coplanar relationship with respect to the second portion.

Abstract: Tools (100) are provided for use in uncapping one or more of a plurality of linearly-oriented, spaced-apart tubes; each cap having a cap upper surface with a depression. The tools comprise a body (30) having first portion (40) for engagement by a user and a second portion (50) for engaging a plurality of caps. The second portion comprises a plurality of spaced-apart projections (60). Each projection is configured for releasably engaging a cap. The projection may comprise two or more spaced-apart projection elements (62,64). Optionally, the first portion is configured in a non-coplanar relationship with respect to the second portion. Methods of use are also provided.

Abstract: The disclosure provides culture devices and methods useful for detecting acid-producing bacteria in a sample. The devices include a nutrient medium and a pH indicator to detect and differentiate acid-producing microorganisms, such as lactic acid bacteria. Methods of use include detecting or enumerating acid-producing microorganisms. The methods further provide for the detection of gas-producing acid-producing bacteria.

Abstract: The disclosure provides culture devices and methods useful for detecting acid-producing bacteria in a sample. The devices include a nutrient medium and a pH indicator to detect and differentiate acid-producing microorganisms, such as lactic acid bacteria. Methods of use include detecting or enumerating acid-producing microorganisms. The methods further provide for the detection of gas-producing acid-producing bacteria.

Abstract: A tool (100) is provided for use in uncapping a plurality of linearly-oriented, spaced-apart tubes; the tubes capped with a unitary closure device comprising a plurality of spaced-apart, linearly-oriented alternating caps and openings, each cap having a cap upper surface and being connected to at least one adjacent cap by at least two connecting structures, each of the at least two connecting structures having a connecting structure upper surface and a connecting structure lower surface, the at least two connecting structures and two adjacent caps forming a boundary of an opening. The tool comprises a body (30) having first portion (42) for engagement by a user and a second portion comprising a base (52) and a plurality of spaced-apart projections (60) extending there-from. Each projection is configured for releasably engaging one of the openings in the unitary closure device. Optionally, the first portion is configured in a non-coplanar relationship with respect to the second portion.

Abstract: Compounds are provided that are either fluorogenic or fluorophoric. Compositions and articles that include the compounds are also provided. Additionally, methods of detecting a microorganism using the compounds are provided. The compounds are fluorinated and can be used advantageously under acidic conditions.

Abstract: Tools (100) are provided for use in uncapping one or more of a plurality of linearly-oriented, spaced-apart tubes; each cap having a cap upper surface with a depression. The tools comprise a body (30) having first portion (40) for engagement by a user and a second portion (50) for engaging a plurality of caps. The second portion comprises a plurality of spaced-apart projections (60). Each projection is configured for releasably engaging a cap. The projection may comprise two or more spaced-apart projection elements (62,64). Optionally, the first portion is configured in a non-coplanar relationship with respect to the second portion. Methods of use are also provided.

Abstract: A latent effervescent body comprising a selective agent is disclosed. A method of using the latent effervescent body in a method to selectively enrich a target microorganism is also disclosed. The method comprises providing a sample, a culture medium, and the latent effervescent body. The method further comprises contacting the sample, the culture medium, and the latent effervescent body under conditions to facilitate growth of the target microorganism. The method further comprises releasing the selective agent from the latent effervescent body. Optionally, the method includes detecting a microorganism.

Abstract: Compositions that comprise water, a first indicator reagent that can be converted by a first biological activity to a first biological derivative, and a plurality of particles are provided. The first indicator reagent can comprise a fluorogenic enzyme substrate having a fluorophore selected from the group consisting of umbelliferone, 7-aminocoumarin, ?-naphthylamine, ?-naphthol, fluorescein, resorufin, 9H-(1,3-dichloro-9,9-dimethyl acridin-2-one), rhodamine 110, a derivative of any of the foregoing fluorophores, and a combination of any of the foregoing fluorophores. The particles are capable of receiving and retaining the first biological derivative from an aqueous liquid. The first biological derivative can be indicative of a microorganism. The compositions further can comprise a gelling agent. Methods of using the compositions to detect the presence or absence of a microorganism in a sample are also provided.

Abstract: The disclosure provides culture devices and methods useful for detecting acid-producing bacteria in a sample. The devices include a nutrient medium and a pH indicator to detect and differentiate acid-producing microorganisms, such as lactic acid bacteria. Methods of use include detecting or enumerating acid-producing microorganisms. The methods further provide for the detection of gas-producing acid-producing bacteria.

Abstract: The invention relates to methods of capturing bacterial whole cells that includes the use of one or more antibodies having antigenic specificities for one or more distinct analytes characteristic of the specific bacterium, followed by analyzing the target whole cells using a direct or indirect ATP assay.

Abstract: Methods for detecting target biological analytes within sample material using acousto-mechanical energy generated by a sensor are disclosed. The acousto-mechanical energy may be provided using an acousto-mechanical sensor, e.g., a surface acoustic wave sensor such as, e.g., a shear horizontal surface acoustic wave sensor (e.g., a LSH-SAW sensor). The detection of the target biological analytes in sample material are enhanced by contacting the target biological analyte and/or the sensor surface with liposomes that amplify the sensor sensitivity by (1) modifying the rheological properties of the fluid near the sensor surface; (2) changing the mass attached to the surface; and/or (3) modifying the dielectric properties of the fluid near the sensor surface, the sensor surface itself and/or any intervening layers on the sensor surface.

Abstract: In certain embodiments, the invention relates to methods of capturing bacterial whole cells that includes the use of two or more antibodies having antigenic specificities for two or more distinct analytes characteristic of the specific bacterium. In certain embodiments, the invention relates to methods of analyzing a sample for a bacterium of interest. In particular, the methods are useful for detecting one or more analytes characteristic of a bacterium of interest, such as components of cell walls that are characteristic of a bacterium, particularly Staphylococcus aureus.