Patents by Inventor Jason W. Holder

Jason W. Holder has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11326143
    Abstract: A method for preparing and processing a sample is provided. The method includes obtaining a sample including biofluid. The method further includes purifying at least part of the sample via an acoustic separator to separate target cells from the sample. The sample may accordingly be at least partially purified. The method further includes causing a portion of an output collected from the acoustic separator to flow through a filter. At least one reagent, such as a lysis reagent or assay reagent, is caused to flow over the cells.
    Type: Grant
    Filed: November 15, 2019
    Date of Patent: May 10, 2022
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Parker Dow, Nicolas Mesyngier, Ken Kotz, Georgiana Kourepenos, Jason O. Fiering, Jason W. Holder
  • Patent number: 10913987
    Abstract: The system described herein for bacterial identification can be used as a point-of-care or lab-based diagnostic system. In some implementations, the system can be used to detect other foreign agents within blood or other samples. The system can include disposable microfluidic cartridges that are removable from the system. The microfluidic cartridges can receive a sample, such as a blood sample, that is suspected of containing bacterial cells and separate the bacterial cells from the blood sample. Once the bacterial cells are separated from the blood, the system can introduce the recombinant detector bacteriophages into the system that can infect the bacterial cells. The system can then detect the expression of reporter genes from the recombinant detector bacteriophages.
    Type: Grant
    Filed: March 27, 2017
    Date of Patent: February 9, 2021
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Jason W. Holder, Jason O. Fiering
  • Publication number: 20200157491
    Abstract: A method for preparing and processing a sample is provided. The method includes obtaining a sample including biofluid. The method further includes purifying at least part of the sample via an acoustic separator to separate target cells from the sample. The sample may accordingly be at least partially purified. The method further includes causing a portion of an output collected from the acoustic separator to flow through a filter. At least one reagent, such as a lysis reagent or assay reagent, is caused to flow over the cells.
    Type: Application
    Filed: November 15, 2019
    Publication date: May 21, 2020
    Inventors: Parker Dow, Nicolas Mesyngier, Ken Kotz, Georgiana Kourepenos, Jason O. Fiering, Jason W. Holder
  • Publication number: 20190048325
    Abstract: The present disclosure provides methods of generating recombinant bacteriophage genomes via semi-synthesis. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.
    Type: Application
    Filed: August 7, 2018
    Publication date: February 14, 2019
    Inventors: Kirsty A. McFarland, Miles T. Rogers, Connor McBrine, Jason W. Holder
  • Publication number: 20180265833
    Abstract: According to various aspects and embodiments, an integrated sensor system is provided. In one example, such an integrated sensor system includes at least one unit cell, an analysis device, and a controller. The at least one unit cell may include an activation chamber containing an activation fluid, a biological chamber containing a biological component, a first membrane disposed in between the activation chamber and the biological chamber, an assay chamber configured to receive a sample, and a second membrane disposed in between the assay chamber and the biological chamber.
    Type: Application
    Filed: September 2, 2016
    Publication date: September 20, 2018
    Inventors: Jason W. Holder, Robert A. Larsen, John Fumo, John Coppeta, Ricky Soong, Amanda N. Billings-Siuti
  • Publication number: 20170298456
    Abstract: The system described herein for bacterial identification can be used as a point-of-care or lab-based diagnostic system. In some implementations, the system can be used to detect other foreign agents within blood or other samples. The system can include disposable microfluidic cartridges that are removable from the system. The microfluidic cartridges can receive a sample, such as a blood sample, that is suspected of containing bacterial cells and separate the bacterial cells from the blood sample. Once the bacterial cells are separated from the blood, the system can introduce the recombinant detector bacteriophages into the system that can infect the bacterial cells. The system can then detect the expression of reporter genes from the recombinant detector bacteriophages.
    Type: Application
    Filed: March 27, 2017
    Publication date: October 19, 2017
    Applicant: The Charles Stark Draper Laboratory, Inc.
    Inventors: Jason W. Holder, Jason O. Fiering
  • Patent number: 8679782
    Abstract: The invention relates to production of triacylglycerols in cells.
    Type: Grant
    Filed: June 15, 2010
    Date of Patent: March 25, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Anthony John Sinskey, Daniel MacEachran, Kazuhiko Kurosawa, Paolo Boccazzi, Jason W. Holder
  • Publication number: 20120171735
    Abstract: The invention relates to production of triacylglycerols in cells.
    Type: Application
    Filed: June 15, 2010
    Publication date: July 5, 2012
    Applicant: Massachusetts Institute of Technology
    Inventors: Anthony John Sinskey, Daniel MacEachran, Kazuhiko Kurosawa, Paolo Boccazzi, Jason W. Holder