Abstract: The disclosure provides methods of making a tetracycline inducible expression system in a cell. The methods include providing the cell with a first nucleic acid sequence comprising a first promoter operably linked to a tetracycline repressor gene coding sequence, providing the cell with a second nucleic acid sequence comprising a second promoter operably linked to a coding sequence of a gene of interest wherein the second promoter is modified to include one or more tetracycline repressor protein binding sites, and determining the expression of the gene of interest in the presence or absence of tetracycline. The disclosure further provides nucleic acid sequences, vectors and cells including the tetracycline inducible modified promoter.

Abstract: A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.

Abstract: The disclosure provides methods of making a tetracycline inducible expression system in a cell. The methods include providing the cell with a first nucleic acid sequence comprising a first promoter operably linked to a tetracycline repressor gene coding sequence, providing the cell with a second nucleic acid sequence comprising a second promoter operably linked to a coding sequence of a gene of interest wherein the second promoter is modified to include one or more tetracycline repressor protein binding sites, and determining the expression of the gene of interest in the presence or absence of tetracycline. The disclosure further provides nucleic acid sequences, vectors and cells including the tetracycline inducible modified promoter.

Abstract: A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.

Abstract: An aerial system is formed by a main closed circuit with diverse stations on both sides of the circuit, along which the production units move hanging on carriages having means of identification, control cards for the status and the main circuit. The main circuit includes a series of identical strips, articulated on primary supports that have a holding part and a trigger to support the carriages with hanging lightweight components. The carriages have a cylindrical holder on the top to hang the primary supports on the wheel triggers. The carriages enter the lowest point at the stations, move up to the main circuit by means of a hoist mechanism which includes a carriage presence detector at the lowest point.

Abstract: Device comprising: a chassis (1) which supports the entire assembly, a telescopic column (2) which comprises a lower fixed portion (5) rotating with respect to a vertical axis, and at least one upper mobile portion (6), a telescopic arm (3) that moves along the vertical column and that supports an x-ray emitter (4) at its end, wherein the telescopic arm-head assembly can be moved from the lower position of the mobile column in its retracted position to the upper position of the mobile column in its extended position, and also in that all the movements of the column are manual and have a mechanical balancing mechanism which comprises a first mechanism consisting of a spring, a block and tackle, and a variable radius pulley all housed in the fixed portion of the column, and a second balancing mechanism consisting of a recovery pulley and a two-radii pulley which balances the weight between the input of the cable and that of the telescopic arm and head assembly.