Abstract: A flexible container is provided of flexible film, the interior surface of the container being fluoropolymer, the container being applicable to the storage and/or preparation of protein separation solution used in the chromatographic purification of protein, such as therapeutic protein.

Abstract: A process for the production of therapeutic protein is provided, which includes the steps of preparing nutrient medium for culturing of cells to express the protein, culturing the cells in the presence of the nutrient medium to express the protein, preparing protein separation solution for isolating the protein, formulating the isolated protein, and storing the formulated protein, at least three of these steps being carried out in separate disposable containers made of flexible film, at least the interior of said surface of said container being made of fluoropolymer.

Abstract: A package is provided, comprising a fluoropolymer bag and an overwrap, the package being terminally sterilized so as to obtain and maintain the sterilization condition of the fluoropolymer bag, which can then be made available as a sterilized, disposable vessel for biological material and corrosive reagents.

Abstract: Therapeutic protein is formulated in a container made of flexible film, at least the interior surface of said container being fluoropolymer, which is preferably terminally sterilized in a sealed overwrap so as to be available for the formulating step in sterilized condition.

Abstract: The manufacture of cellular product such as a protein by expression from a cell line in a bioreactor is carried out wherein the bioreactor is a container made of flexible film, at least the interior surface of the container being fluoropolymer, and in addition, the nutrient medium and other agents used in the fermentation or cell culture process can be prepared in a separate container of the same film.

Abstract: A pelvic traction harness including a circumferential belt that is adapted to be secured around the torso of a patient and has a first end, a second end and a posterior area intermediate the first end and the second end. The harness also includes a separate cinching strap belt that is adapted to be secured around the circumferential belt and has a male end, a female end and an anterior region intermediate the male end and the female end. The harness also includes a pulling strap having an anterior end and a posterior end, said anterior end being adapted to be attached to anterior region of the cinching strap belt, said posterior end being adapted to be attached to the posterior area of the circumferential belt. The pulling strap is also adapted to extend between the legs of the patient and attach to a source for applying a pulling force. The invention also includes a method for applying a traction force to the spine of a patient through the pelvic region of the patient.