Abstract: A method for providing information to a customer who is requesting a synthesized oligonucleotide, peptide, peptide nucleic acid, polysaccharide or antibody (collectively “strands”). The capabilities of one or more suppliers are stored and automatically searched for capabilities to synthesize the requested strand. A list of the suppliers with the required capabilities is generated along with the price of the strand. The invention also encompasses an apparatus for providing information to remote users regarding which suppliers can supply a desired strand. The apparatus has a database and a server which allows the remote user to access the capabilities of one or more suppliers for synthesizing a strand.

Abstract: Active recombinant human IL-4 is purified from a crude cell culture medium of a CHO-cell lines mutant by subjecting said crude solution to two sequentialcation exchange chromatographies at pH 7.2 and 0.12M sodium chloride, selective chromatography on a cobalt-chelating agarose column at pH 7.2 in a buffer containing a high concentration of sodium chloride, i.e., about 0.5M sodium chloride, further treated by concentration (diafiltration) up to 20 mg/mL and size exclusion gel chromatography using a citrate buffer at pH 4.5.