Abstract: Provided herein are systems and methods for isolating a target from a biological sample. In some aspects, provided herein are automated systems and methods for isolating a target from a plurality of biological samples. For example, provided herein are automated systems and methods for isolating viral nucleic acid from a biological sample using a magnetic force to draw the nucleic acid out of a liquid biological sample.

Abstract: Provided herein are devices and methods for isolation and detection of an analyte from a sample. In some embodiments, provided herein are devices and methods of use thereof for vertical flow-based isolation and detection of analytes in a liquid sample, such as a urine sample.

Abstract: Provided herein are mobile systems for sample processing. In some aspects, provided herein are mobile systems for sample processing and methods of use thereof for detection of pathogens in biological samples.

Abstract: Systems, devices, compositions and methods for positioning and/or processing a target are provided. The invention includes systems, devices, methods and related compositions useful, for example, for the separation, isolation, purification, identification, detection and quantification of materials. Also provided are systems and methods for isolation, and/or detection and/or quantification of a target or analyte in a sample. Some systems, devices, compositions and methods comprise oil and aqueous phases stabilized in close proximity to each other. Some systems, devices and methods use a magnetic force to draw a target or carrier-bound through multiple layers. In some embodiments, systems and devices comprise reagents for detection of a target or analyte.

Abstract: Stabilized interface systems and compositions for positioning target(s), comprising fluids, associated structural material(s) having a pore that permits passage and positioning of targets and related compositions, and a fluid phase, layer, or interface stabilized with the associated structural material. Miscible interface systems and compositions for positioning target(s) for detection comprising two or more fluid regions with different properties within a phase or layer where the fluid regions are stabilized with respect to each other using a solid or semi-solid structure or material with at least one pore that allows passage of said target(s) wherein stabilization allows mass transport of a fluid constituent via diffusion to prevail over bulk fluid motion.

Abstract: Provided herein are devices, systems, and methods for concentration and detection of sample components. In particular, provided herein are lateral flow devices, systems, and methods that utilize flow control of samples.

Abstract: A device and method of laminar flow patterning of at least one sample fluid in a main channel in a microfluidic device are provided. A first input channel is provided in the microfluidic device. The first input channel has an output end communicating with the first end of the main channel and an input end communicating with a first input port. A buffer fluid is deposited in the main channel and the first input channel and a first sample fluid is deposited in the first input port. A first pressure is generated in response to the depositing of the first sample fluid in the first input port so as to cause laminar flow of the first sample fluid in the main channel.

Abstract: A method is provided for collecting a concentration of particles from a sample fluid containing the particles. The method includes the steps of providing a microfluidic device. The microfluidic device includes an input channel, an output channel and a collection region. The input channel has an input end and an output end. The output channel has an input end and an output end. The collection region interconnects the output end of the input channel and the input end of the output channel. The sample fluid flows through the input channel and the output channel at a first velocity and through the collection region at a second velocity less than the first velocity such that the particles collect in therein.

Abstract: A method and device are provided for actuating fluid flow in a channel of a microfluidic device. The microfluidic device has a first and second ports communicating with the channel. The channel is filled with a fluid and oscillatory movement of the fluid in the channel is generated in response to movement of an actuator between a first retracted position and a second extended position.

Abstract: A device and method of laminar flow patterning of at least one sample fluid in a main channel in a microfluidic device are provided. A first input channel is provided in the microfluidic device. The first input channel has an output end communicating with the first end of the main channel and an input end communicating with a first input port. A buffer fluid is deposited in the main channel and the first input channel and a first sample fluid is deposited in the first input port. A first pressure is generated in response to the depositing of the first sample fluid in the first input port so as to cause laminar flow of the first sample fluid in the main channel.

Abstract: A method is provided for collecting a concentration of particles from a sample fluid containing the particles. The method includes the steps of providing a microfluidic device. The microfluidic device includes an input channel, an output channel and a collection region. The input channel has an input end and an output end. The output channel has an input end and an output end. The collection region interconnects the output end of the input channel and the input end of the output channel. The sample fluid flows through the input channel and the output channel at a first velocity and through the collection region at a second velocity less than the first velocity such that the particles collect in therein.