Abstract: A method for receiving, distributing and storing a sample into numerous aliquots of small volume without air entrapment and with retention of aliquots when the device is manipulated and for the treatment of any or all of the aliquots with the same or different reagents and/or other chemical additives.

Abstract: The present invention is a device which allows for receiving, distributing and storing a sample into numerous aliquots of small volume without air entrapment and with retention of aliquots when the device is manipulated. The device allows for the treatment of any or all of the aliquots with the same or different reagents and/or other chemical additives. The device comprises a housing for containing a body for guiding a sample into a plurality of wells without the need for pipetting aids, without multiple manipulations, without the retention of air and for retaining sample in the wells.

Abstract: Acid-fast bacilli are stained with a primary stain that does not require heating and then with a decolorizing counter-stain that does not require a separate acid or alcohol decolorization step. The primary stain includes basic fuchsin, ethyl alcohol, isopropyl alcohol, phenol and water. The counter-stain includes methylene blue, ethyl alcohol, potassium hydroxide, glycerol, glacial acetic acid, polyvinylpyrrolidone and water.