Abstract: The present invention relates to a method of obtaining altered plasmid contents in bacteria, bearing mutation in at least one of the chromosomal genes, nusG, rho, and dnaC, and the bacterial strains thereof, having the mutated chromosomal genes, individually or in various possible combinations, capable of altering the level of plasmids.

Abstract: The present invention relates to a method of obtaining altered plasmid contents in bacteria, bearing mutation in at least one of the chromosomal genes, nusG, rho, and dnaC, and the bacterial strains thereof, having the mutated chromosomal genes, individually or in various possible combinations, capable of altering the level of plasmids.

Abstract: The present invention relates to a method of obtaining altered plasmid contents in bacteria, bearing mutation in at least one of the chromosomal genes, nusG, rho, and dnaC, and the bacterial strains thereof, having the mutated chromosomal genes, individually or in various possible combinations, capable of altering the level of plasmids.

Abstract: The present invention relates to a method of obtaining altered plasmid contents in bacteria, bearing mutation in at least one of the chromosomal genes, nusG, rho, and dnaC, and the bacterial strains thereof, having the mutated chromosomal genes, individually or in various possible combinations, capable of altering the level of plasmids.

Abstract: The invention relates to a process for determining the frequency of mutations in living and essentially nondividing cells; a process for obtaining strains of a particular specified genotype in which such mutation frequencies may be determined; and a process for determining the effects of various agents including chemical substances and genes on the frequency of mutations in living and essentially nondividing cells.

Abstract: The invention relates to the construction and use of novel expression systems that make use of DNA sequences encoding a promoter or promoters along with cis-regulatory elements, such as those for proU, that permit osmotically inducible initiation of transcription in bacteria; the expression systems of the present invention can be used to efficiently hyperexpress heterologous gene products including proteins or polypeptides; methods for construction of vectors and strains enabling NaCl-induced hyperexpression of heterologous gene products in several organisms such as Escherichia coli, and methods for NaCl-induced hyperexpression of heterologous gene products, including proteins or polypeptides, using the said novel combinations in a variety of organisms such as Escherichia coli, and further purification of the gene product of interest in either laboratory or industrial scale production systems.