Abstract: The invention relates to a method for diagnosing or identifying pancreas cancer in a subject. The inventors indeed identified two DNA methylation biomarkers that, alone, preferably in combination, can help diagnosing or following-up pancreatic cancer patients very specifically, discriminating with other type of cancers. Further, it can be used for determining, adapting a suitable therapeutic regimen for a subject diagnosed for pancreas cancer. The present invention also relates to kit comprising primers or probes to detect, diagnose, identify hypermethylated genes.