Abstract: The present invention relates to compositions and methods for raising antibodies generally comprising 1) providing highly immunogenic vesicles bearing at least one target antigen and 2) immunizing animals with the said antigen-bearing vesicles to induce antigen-specific antibody responses. The invention also relates to methods of screening antibody repertoires comprising 1) providing vesicles bearing at least one target antigen and one marker and 2) isolating antibody-producing cells or particles with defined antigen specificity using the said antigen- and marker-bearing vesicles. Antibodies with defined antigen specificity can then be prepared from isolated antibody-producing cells using known methods of the art. This invention can be used in experimental, research, therapeutic, prophylactic or diagnostic areas.

Abstract: The present invention relates to compositions and methods for selectively expressing a polypeptide in a membrane vesicle. The invention also relates to genetic constructs and recombinant cells suitable to produce such membrane vesicles. This invention also relates to such functionalized membrane vesicles as well as to methods of making antibodies, methods of producing or regulating an immune response as well as to methods of screening or identifying binding partners using the same. The invention more particularly uses lactadherin or portions thereof to selectively express polypeptides in membrane vesicles, of natural or synthetic origin. This invention can be used in experimental, research, therapeutic, prophylactic or diagnostic areas.

Abstract: The present invention relates to exosome-specific ligands and compositions comprising the same. The invention also relates to methods of generating said ligands and compositions, to methods of using said ligands or compositions, e.g., to block the exosome pathway or to detect and/or characterize exosomes in a sample or subject, as well as to the antigens contacted by said ligands or compositions. The application can be used in experimental, research, therapeutic, prophylactic or diagnostic areas.

Abstract: The present invention relates to compositions and methods for raising antibodies generally comprising 1) providing highly immunogenic vesicles bearing at least one target antigen and 2) immunizing animals with the said antigen-bearing vesicles to induce antigen-specific antibody responses. The invention also relates to methods of screening antibody repertoires comprising 1) providing vesicles bearing at least one target antigen and one marker and 2) isolating antibody-producing cells or particles with defined antigen specificity using the said antigen- and marker-bearing vesicles. Antibodies with defined antigen specificity can then be prepared from isolated antibody-producing cells using known methods of the art. This invention can be used in experimental, research, therapeutic, prophylactic or diagnostic areas.

Abstract: The present invention relates to compositions comprising vesicles released from activated T lymphocytes, as well as to methods for their production and uses. Said vesicles contain a set of bioactive molecules which confer remarkable properties, such as antigen recognition, antigen presentation and other regulatory and effector functions. This invention also relates to methods for transferring or delivering antigenic molecules (e.g., peptides, peptide/MHC complexes, TCR or subunit thereof, etc.) to antigen presenting cells (APCs) using said vesicles, to induce specific immune responses, particularly specific CTL responses. The invention further relates to methods of delivering molecules selectively or specifically to target cells using said vesicles.

Abstract: The present invention relates to methods of preparing biological material, for use in various experimental, diagnostic or therapeutic uses, including immunotherapy treatment or prophylaxy of tumors. More particularly, the present invention relates to methods of preparing membrane vesicles (in particular exosomes) released by various types of mammalian cells, comprising diafiltration and/or density cushion centrifugation. The invention also provides novel methods for characterizing and analyzing exosome preparations, which can be used in quality control assay for the purpose of pharmaceutical product production. The invention is suitable to produce pharmaceutical grade preparations of such membrane vesicles and to fully characterize said preparations, for use in human beings.

Abstract: The present invention relates to methods of preparing biological material, for use in various experimental, diagnostic or therapeutic uses, including immunotherapy treatment or prophylaxy of tumors. More particularly, the present invention relates to methods of preparing membrane vesicles (in particular exosomes) released by various types of mammalian cells, comprising diafiltration and/or density cushion centrifugation. The invention also provides novel methods for characterizing and analyzing exosome preparations, which can be used in quality control assay for the purpose of pharmaceutical product production. The invention is suitable to produce pharmaceutical grade preparations of such membrane vesicles and to fully characterize said preparations, for use in human beings.

Abstract: The present invention relates to compositions and methods for selectively expressing a polypeptide in a membrane vesicle. The invention also relates to genetic constructs and recombinant cells suitable to produce such membrane vesicles. This invention also relates to such functionalized membrane vesicles as well as to methods of making antibodies, methods of producing or regulating an immune response as well as to methods of screening or identifying binding partners using the same. The invention more particularly uses lactadherin or portions thereof to selectively express polypeptides in membrane vesicles, of natural or synthetic origin. This invention can be used in experimental, research, therapeutic, prophylactic or diagnostic areas.

Abstract: Derivatives of 9-hydroxy ellipticine, preparation thereof, and use thereof for the treatment of leukemias and solid tumors.

Abstract: Derivatives of 9-hydroxy ellipticine, preparation thereof, and use thereof for the treatment of leukemias and solid tumors.

Abstract: 9-hydroxy ellipticine, preparation thereof, and use thereof for the treatment of leukemias and solid tumors.