Abstract: Genetically modified gram-negative bacteria, in particular E. coli, that are oversensitive to lysis. These bacteria are therefore useful to improve the yield of nucleic acid extraction, preferably extra-genomic nucleic acid extraction (e.g. plasmid), and/or the yield of a polypeptide, preferably encoded by an extra-genomic nucleic acid. In practice, the strains of E. coli are engineered with a combination of at least 2 mutated genes altering the envelop integrity. More particularly, at least one mutated gene is ompA and at least one mutated gene is a gene involved in Lpp functionality, such as, e.g., the lpp gene, the ybiS gene, the ycfS gene and the erfK gene. These combinations also include mutations in genes that are homologue to the ompA gene, and/or the lpp gene.

Abstract: A processing method of content intended for broadcasting is disclosed. The method includes, preceding broadcasting: encryption of the content by a plurality of control words, reservation of locations intended to receive a control message in the encrypted content, marking of each location with location information in the content, and storage of a key file comprising the control words. During broadcasting, the method includes: transmission of the encrypted content to a video server, transmission of the key file to a control message generator, broadcasting of the content encrypted by the video server, interception of the video server output by a replacement module, detection, by the replacement module, of location information of control messages in the stream of the broadcasted content, and application of the control messages at locations provided for this purpose by the replacement module.

Abstract: A processing method of content intended for broadcasting is disclosed. The method includes, preceding broadcasting: encryption of the content by a plurality of control words, reservation of locations intended to receive a control message in the encrypted content, marking of each location with location information in the content, and storage of a key file comprising the control words. During broadcasting, the method includes: transmission of the encrypted content to a video server, transmission of the key file to a control message generator, broadcasting of the content encrypted by the video server, interception of the video server output by a replacement module, detection, by the replacement module, of location information of control messages in the stream of the broadcasted content, and application of the control messages at locations provided for this purpose by the replacement module.

Abstract: The aim of this invention is to propose a method allowing specific services to be received by a user A from a broadcaster in which this user is not initialised.