Abstract: A device (10) for optical examination of biological materials (68) using radiation of a selected wavelength includes a substrate (50) having a first surface and a second surface opposite to the first surface. The first surface includes a dense array of micro-optical elements (54) arranged to provide increased intensity radiation or evanescent radiation. The first surface is in close proximity to the biological material (68) being examined.

Abstract: Fluid is deposited in spots using supported, compliant pin arragements, supplied by a local reservoir. Pin arragements in the form of reciprocating and rotating devices are shown. Supply of the pins by mobile local subreservoirs limit the range of travel between drop-pickup and drop deposit. Local reservoirs in the form of circular rings and large pins are disclosed. Compliance is achieved by using spring and flexure arrangements. Some embodiments employ planer flexures to mount the pin and constrict its movement. Such deposit techniques are shown used in many analytical, reactive, and productive conditions. Combination of the use of the versatile, high density array or with a flying mini objective, wide field scanning microscope is disclosed. Combination with other sub systems adds to the versatility of the system.

Abstract: For depositing fluid dots in an array, e.g., for microscopic analysis, a deposit device, e.g. a pin, cooperating with a fluid source defines a precisely sized drop of fluid of small diameter on a drop carrying surface. Transport mechanism positions the device precisely over the receiving surface and drive mechanism moves the deposit device toward and away from the surface. By repeated action, minute drops of fluid can be deposited precisely in a dense array, preferably under computer control. The drop-carrying surface shown has a diameter less than 375, preferably less than 300, preferably between about 15 and 250 micron, and is bound by a sharp rim that defines the perimeter of the fluid drop. The deposit device is compliant in the direction of deposition motion, e.g. by overcoming resistance of a resilient member. When depositing, the deposit device is laterally constrained to a reference position, e.g.

Abstract: An optical apparatus for examination of biological material, a light source, a light detector and several optical elements for providing light from the light source to an optical substrate. The optical substrate having a surface comprising a dense array of micro-optical elements in close proximity to biological material being examined. The optical apparatus may include optical elements such as a lens that is arranged to provide light to and collect light from the dense array of micro-optical elements and the biological material. The lens and the dense array of micro-optical elements provide optical properties that increase the number of photons interacting with the biological material or increase the number of photons collected by the lens after interaction with the biological material.

Abstract: An attachment device is described for attaching a part to a structure. The part may be, for example, an optical element such as a lens or a mirror, that is to be attached, for example, to an optical instrument such as a scanner used to scan biological materials. The device includes a base and two or more holding elements coupled to the base. The holding elements may include a first group of two or more opposing holding elements of a first length and a second group of two or more opposing holding elements of a second length shorter than the first length. Each holding element flexibly engages at least one surface of the part when the device is engaged with the part. A third group of two or more opposing holding elements may also be provided that also are shorter than the first length and that rigidly engage the part. The base and the holding elements may be formed of a single piece of flexible material.

Abstract: An apparatus for depositing an array of spots on a receiving surface includes a fluid source, a deposit device that includes a drop-carrying surface, and a cleaning device. The deposit device and a fluid source are cooperatively arranged to deposit a spot(usually created by a drop) on a receiving surface. The cleaning device is constructed and arranged to clean the drop-carrying surface by employing a flow of cleaning fluid or another cleaning matter such as small particles the flow being arranged to prevent contaminating back flow.

Abstract: An optical scanning system for examining material associated with a substrate includes at least one scanning module for displacing two or more objective lenses, at least one optical coupling system and a translation system. The two objective lenses are mounted on one or more scan arms and are constructed to scan over regions or subregions associated with the substrate. The scanning module is configured to displace the scan arm(s) to perform the scan and thereby displace the two objective lenses. Each objective lens is arranged to deliver light to the material and collect light from the material.

Abstract: Microscopes, including viewing and other microscopic systems, employ a hinged, tiltable plate to adjust focus on a flat object such as a microscope slide or biochip by motion, achieved by tilting, which is substantially normal to the focus point on the plane of the object. By employing two such tiltable arrangements, relatively long scan lines of e.g., flying objective, single pixel on-axis scanning can be accommodated. The tilting support plate is specifically constructed to provide tailored locations for different objects in series along the Y axis of the plate. The plate can accommodate heaters and cooled plates and/or the flat object being examined. In a fluorescence scanning microscope, locations are specifically adapted to receive microscope slides and biochip cartridges such as Affymetrix's “Gene Chip®”. A scanning microscope under computer control, employing such a focusing action, enables unattended scanning of biochips with a simple and economical instrument.

Abstract: A high torque electromagnetic device such as a moving magnet galvanometer includes a composite electromagnetic coil. The composite electromagnetic coil includes two pancake-shaped coil parts made using a rectangular conductor having a width and a thickness, wherein the winding formed for each pancake-shaped coil part has the width oriented in a direction substantially normal to the initially flat face of the pancake-shaped coil part. Each pancake-shaped coil part is subsequently shaped to a shape different from the initially flat shape. The coil parts are superposed and electrically connected to form a single tightly packed unit.

Abstract: The present invention relates to calibration apparatuses, methods or tools used in microscopy. A calibration tool for fluorescent microscopy includes a support, a solid surface layer including a fluorescent material, and a thin opaque mask of non-fluorescent material defining reference feature openings having selected dimensions exposing portions of the surface layer. A first type of the calibration tool may include an adhesion promoter facilitating contact between the surface of the support and the solid surface layer including the fluorescent material, which is in contact with the thin opaque mask. A second type of the calibration tool may include the thin opaque mask fabricated (with or without an adhesion promoter) onto the support, and the solid surface layer including the fluorescent material located on the thin opaque mask.

Abstract: A calibration tool for fluorescent microscopy comprises a support on which is carried a solid surface layer comprised of effective fluorophores, and a thin mask of non-fluorescent material defining reference feature openings of limited dimensions exposes portions of the fluorophore-comprising surface layer.

Abstract: For depositing fluid dots in an array, e.g., for microscopic analysis, a deposit device, e.g. a pin, cooperating with a fluid source defines a precisely sized drop of fluid of small diameter on a drop carrying surface. Transport mechanism positions the device precisely over the receiving surface and drive mechanism moves the deposit device toward and away from the surface. By repeated action, minute drops of fluid can be deposited precisely in a dense array, preferably under computer control. A mobile-fluid storage device resupplies the deposit device along the array, e.g. in the immediate vicinity of the deposit locations. Mobile annular storage rings are lowered and raised to obtain a supply of fluid, or a mobile multiwell plate is used. Cleaning mechanism is shown, in particular a jet arrangement that directs a jet from a pin or tool axis portal to deeper into the confinement chamber, to scrub along a pin or pin-like structure while inducing an air flow from the working zone to prevent back contamination.

Abstract: For depositing fluid dots in an array, e.g., for microscopic analysis, a deposit device, e.g. a pin, cooperating with a fluid source defines a precisely sized drop of fluid of small diameter on a drop carrying surface. Transport mechanism positions the device precisely over the receiving surface and drive mechanism moves the deposit device toward and away from the surface. By repeated action, minute drops of fluid can be deposited precisely in a dense array, preferably under computer control. The drop-carrying surface shown has a diameter less than 375, preferably less than 300, preferably between about 15 and 250 micron, and is bound by a sharp rim that defines the perimeter of the fluid drop. The deposit device is compliant in the direction of deposition motion, e.g. by overcoming resistance of a resilient member. When depositing, the deposit device is laterally constrained to a reference position, e.g.

Abstract: An optical scanning system for examining material associated with a substrate includes at least one scanning module for displacing two or more objective lenses, at least one optical coupling system and a translation system. The two objective lenses are mounted on one or more scan arms and are constructed to scan over regions or subregions associated with the substrate. The scanning module is configured to displace the scan arm(s) to perform the scan and thereby displace the two objective lenses. Each objective lens is arranged to deliver light to the material and collect light from the material.

Abstract: Microscopes, including viewing and other microscopic systems, employ a hinged, tiltable plate to adjust focus on a flat object such as a microscope slide or biochip by motion, achieved by tilting, which is substantially normal to the focus point on the plane of the object. By employing two such tiltable arrangements, relatively long scan lines of e.g., flying objective, single pixel on-axis scanning can be accommodated. The tilting support plate is specifically constructed to provide tailored locations for different objects in series along the Y axis of the plate. The plate can accommodate heaters and cooled plates and/or the flat object being examined. In a fluorescence scanning microscope, locations are specifically adapted to receive microscope slides and biochip cartridges such as Affymetrix's “Gene Chip®”. A scanning microscope under computer control, employing such a focusing action, enables unattended scanning of biochips with a simple and economical instrument.

Abstract: An attachment device is described for attaching a part to a structure. The part may be, for example, an optical element such as a lens or a mirror, that is to be attached, for example, to an optical instrument such as a scanner used to scan biological materials. The device includes a base and two or more holding elements coupled to the base. The holding elements may include a first group of two or more opposing holding elements of a first length and a second group of two or more opposing holding elements of a second length shorter than the first length. Each holding element flexibly engages at least one surface of the part when the device is engaged with the part. A third group of two or more opposing holding elements may also be provided that also are shorter than the first length and that rigidly engage the part. The base and the holding elements may be formed of a single piece of flexible material.

Abstract: A high torque electromagnetic device such as a moving magnet galvanometer includes a composite electromagnetic coil. The composite electromagnetic coil includes two pancake-shaped coil parts made using a rectangular conductor having a width and a thickness, wherein the winding formed for each pancake-shaped coil part has the width oriented in a direction substantially normal to the initially flat face of the pancake-shaped coil part. Each pancake-shaped coil part is subsequently shaped to a shape different from the initially flat shape. The coil parts are superposed and electrically connected to form a single tightly packed unit.

Abstract: A fluid deposit assembly mounted on a carrier for depositing minute drops of fluid at selected locations upon a substrate, comprising a deposit element having an exposed tip of diameter of 0.3 mm or less constructed and arranged to carry and deposit drops of fluid upon the substrate, stable lateral reference surfaces or surface portions exposed for engagement by the deposit element, the surfaces or surface portions being constructed and arranged to prevent X, Y displacement of the deposit element relative to the carrier when the deposit element is urged thereagainst and design for urging the deposit element against the reference surfaces or surface portions at least at the time that the deposit element approaches a substrate to deposit a fluid drop. The reference surfaces or surface portions and the design for urging are cooperating to precisely position the deposit tip in a precisely desired position as it contacts the substrate. The deposit element is shown as the tip of an axially moveable pin.

Abstract: Microscopes, including viewing and other microscopic systems, employ a hinged, tiltable plane to adjust focus on an object such as a microscope slide. A scanning microscope under computer control, employing such a focusing action, enables unattended scanning of biochips with a simple and economical instrument. Also shown are flexure-mounting of a support plate to define the hinge axis, techniques for automatically determining position and focus, and a rotatably oscillating flying micro-objective scanner combined with the tilting plane focus system. Construction and control techniques are shown that realize simple and accurate focusing. Methods of examination of biological materials are disclosed. Simple and efficient focused scanning with a flying micro-objective of ordered arrays of nucleotides and nucleic acid fragments carried upon a microscope slide or other substrate is discovered.

Abstract: For enabling precise operation of an oscillating device, such as a galvanometric optical scanner, a capacitive position transducer is provided with strategically located internal capacitive fiducial features that interact with the armature of the transducer beyond the central range of excursion of the transducer, typically beyond the normal operating range of the device. Electric pulses obtained at instants of interaction with the fiducials enable determination of position drift caused e.g. by change in environmental conditions. The pulses can be detected by simple circuits to produce recalibration of the amplitude and null position of the instrument on an automatic or elective basis.