Abstract: A Brassica sp. genomic clone G 363 containing a gene promoter, which directs expression in the stigma, was isolated. Based on microbombardment studies of Brassica napus flowers with the gene Pis 63/Gus fusion, the Pis 63 promoter was shown to direct GUS expression to the stigma. This promoter will be useful for the temporal and spatial control of gene expression in plants.

Abstract: A Brassica sp. genomic clone containing a polygalacturonase gene promoter was isolated. This promoter directs high levels of transcription in the pollen. When the promoter was fused with the GUS gene and introduced into B. napus by Agrobacterium mediated transformation, this promoter controlled GUS expression in microspores and pollen in transgenic B. napus plants. Expression occurred between the late uninucleate microspore stage and the mature pollen grain stage. GUS activity was also identified in tapetal tissue at the binucleate microspore stage. There was no GUS expression in other tissues such as root, stem, leaf, sepal, petal or pistil. This promoter will be useful for the temporal and spatial control of endogenous gene expression in plants.