Abstract: The present invention relates to a temperature-stable labeling reagent of formula: in which R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers linked together by at least one multimeric structure, L is a linking arm containing a linear succession of at least two covalent bonds and n an integer equal to 0 or 1, R3 and R4 represent independently of each other: H, NO2, Cl, Br, F, I, R2-(L)n-Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR with R=alkyl or aryl, A is a linking arm containing at least one covalent double bond allowing conjugation of the diazo functional group with the aromatic ring and u is an integer between 0 and 2, preferably equal to 0 or 1, and —Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—.

Abstract: A single- or double-stranded deoxyribonucleic acid (DNA) is fragmented and labeled by chemically fragmenting the DNA by creating at least one abasic site on the DNA, and attaching a marker to at least one of the fragments by means of a labeling reagent, the reagent covalently and predominantly coupling to at least one phosphate of the fragment. This method can be applied in the field of diagnosis.

Abstract: The invention relates to a functionalized compound of general formula (I): in which W represents a nucleotide analog, L represents a linker arm comprising at least four atoms, R1 represents a linear or branched alkyl chain, A functionalized polynucleotide comprising at least one said compound and a labeled functionalized polynucleotide comprising at least one functionalized compound corresponding to the formula (I?). A method for detecting a target nucleic acid using a said compound is described.

Abstract: The present invention relates to a temperature-stable labeling reagent of formula: 1

Abstract: The invention relates to a method for labeling and fragmenting a single- or double-stranded deoxyribonucleic acid (DNA) comprising the following steps:

Abstract: A target nucleic acid sequence amplification method wherein at least the target nucleic acid sequence, at least one oligonucleotide primer specific for the target sequence, one or more enzymatic activities and nucleotides are provided, and the target sequence is amplified under conditions suitable in particular for said enzymatic or activity or activities, is disclosed. According to the method, at least one of the nucleotides is a prefunctionalized nucleotide that differs from the other nucleotides at least in that it includes at least one unprotected reactive covalence function in at least one predetermined site of the base of said nucleotide, in order to give a prefunctionalized amplificati product including at least one such prefunctionalized nucleotide. Nucleotides for carrying out the method are also disclosed.