Abstract: The present invention relates to a method for screening compounds for bactericidal activity using thermostable luciferase and based on a real-time bioluminescence measurement. The present invention further relates to a method for determining the sensitivity of a bacterial sample originating from a subject suffering from a bacterial infection to a group of known antibiotics and to a method for assessing the minimum inhibitory concentration (MIC) of a bactericidal compound.

Abstract: The invention relates to an Amycolatopsis strain derived from the Amycolatopsis Zyl 926 strain having at least one additional copy of the FCS and ECH genes encoding feruloyl-CoA-synthetase and enoyl-CoA-hydratase/aldolase integrated at the integration site of the ?C31 phage. The invention also relates to the method for producing such strains, the integration cassette of the FCS and ECH genes, the use of said strains in a method for producing vanillin, and a method for producing vanillin.

Abstract: The invention relates to an Amycolatopsis strain derived from the Amycolatopsis Zyl 926 strain having at least one additional copy of the FCS and ECH genes encoding feruloyl-CoA-synthetase and enoyl-CoA-hydratase/aldolase integrated at the integration site of the ?C31 phage. The invention also relates to the method for producing such strains, the integration cassette of the FCS and ECH genes, the use of said strains in a method for producing vanillin, and a method for producing vanillin.

Abstract: Isolated, natural or synthetic polynucleotide and polypeptide encoded by said polynucleotide, that is involved in the synthesis of cyclodipeptides, recombinant vector comprising said polynucleotide or any substantially homologous polynucleotide, host cell modified with said polynucleotide or said recombinant vector and also methods for in vitro and in vivo synthesizing cyclodipeptides, in particular cyclo(Tyr-Xaa) cyclodipeptides, wherein Xaa is any amino acid and their derivatives and applications thereof.

Abstract: Use of CDSs in the synthesis of linear dipeptides, and applications thereof for the in vivo and in vitro synthesis of linear dipeptides, in particular Phe-Leu, Leu-Phe, Phe-Phe, Phe-Tyr, Tyr-Phe, Leu-Leu, Leu-Tyr, Tyr-Leu, Phe-Met, Met-Phe, Leu-Met, Met-Leu, Tyr-Met, Met-Tyr, Met-Met, Tyr-Tyr, Ile-Met, Met-Ile, Leu-Ile, Ile-Leu using the corresponding polynucleotides.

Abstract: The invention concerns novel isolated natural or synthetic polynucleotides and polypeptides coded by said polynucleotides, involved in the synthesis of diketopiperazine derivatives, vectors comprising said polynucleotides, micro-organisms transformed with said polynucleotides, uses of said polynucleotides and said polypeptides, as well as methods for the synthesis of diketopiperazine derivatives, including cyclodipeptides and diketopiperazine derivatives 3- and 6-substituted by ?,?-unsaturated amino acid side chains.

Abstract: Isolated, natural or synthetic polynucleotide and polypeptide encoded by said polynucleotide, that is involved in the synthesis of cyclodipeptides, recombinant vector comprising said polynucleotide or any substantially homologous polynucleotide, host cell modified with said polynucleotide or said recombinant vector and also methods for in vitro and in vivo synthesizing cyclodipeptides, in particular cyclo(Tyr-Xaa) cyclodipeptides, wherein Xaa is any amino acid and their derivatives and applications thereof.

Abstract: Isolated, natural or synthetic polynucleotides and the polypeptides encoded by said polynucleotides, that are involved in the synthesis of cyclodipeptides, the recombinant vectors comprising said polynucleotides or any substantially homologous polynucleotides, the host cell modified with said polynucleotides or said recombinant vectors and also methods for in vitro and in vivo synthesizing cyclo(Leu-Leu) cyclodipeptide and its derivatives and applications thereof.

Abstract: The invention concerns a method for preparing nucleic acids from an environment sample, more particularly a method for obtaining a library of nucleic acids from a sample. The invention also concerns nucleic acids of nucleic acid libraries obtained by said method their use in the synthesis of novel compounds, in particular novel compounds of therapeutic interest. The invent further concerns novel means used in the method for obtaining said nucleic acids, such as novel vectors and novel processes for preparing such vectors or recombinant host cells containing said nucleic acid. Finally, the invention concerns methods for detecting a nucleic acid of interest within a library of nucleic acids resulting from said method, and nucleic acids detected by said method and polypeptides encoded by said nucleic acids.

Abstract: The invention concerns novel isolated natural or synthetic polynucleotides and polypeptides coded by said polynucleotides, involved in the synthesis of diketopiperazine derivatives, vectors comprising said polynucleotides, micro-organisms transformed with said polynucleotides, uses of said polynucleotides and said polypeptides, as well as methods for the synthesis of diketopiperazine derivatives, including cyclodipeptides and diketopiperazine derivatives 3- and 6-substituted by ?,?-unsaturated amino acid side chains.

Abstract: The invention concerns novel isolated natural or synthetic polynucleotides and polypeptides coded by said polynucleotides, involved in the synthesis of diketopiperazine derivatives, vectors comprising said polynucleotides, micro-organisms transformed with said polynucleotides, uses of said polynucleotides and said polypeptides, as well as methods for the synthesis of diketopiperazine derivatives, including cyclodipeptides and diketopiperazine derivatives 3- and 6- substituted by ?,?-unsaturated amino acid side chains.

Abstract: The invention concerns novel isolated natural or synthetic polynucleotides and polypeptides coded by said polynucleotides, involved in the synthesis of diketopiperazine derivatives, vectors comprising said polynucleotides, micro-organisms transformed with said polynucleotides, uses of said polynucleotides and said polypeptides, as well as methods for the synthesis of diketopiperazine derivatives, including cyclodipeptides and diketopiperazine derivatives 3- and 6- substituted by ?,?-unsaturated amino acid side chains.

Abstract: The present invention relates to the isolation and identification of novel genes of the biosynthetic pathway for spiramycins and to novel polypeptides involved in this biosynthesis. The invention also relates to a method for producing these polypeptides. It also relates to the use of these genes for the purpose of increasing the levels of production and the purity of the spiramycin produced. The invention relates in particular to a microorganism which produces spiramycin I but which does not produce spiramycin II and III, and to the use of such a microorganism. The invention also relates to the use of the genes of the biosynthetic pathway for spiramycins for constructing mutants which can lead to the synthesis of novel antibiotics or to derived forms of spiramycins. The invention also relates to the molecules produced through the expression of these genes and to pharmacologically active compositions of a molecule produced through the expression of such genes.

Abstract: Novel nucleic acid sequences, vectors for expressing same, and uses of said sequences, in particular in actinomycetes fermentation methods.