Patents by Inventor Jean-Marie Buerstedde

Jean-Marie Buerstedde has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Fluorescent GFP variant displaying highly increased fluorescence intensity without a spectral shift
    Patent number: 8742081
    Abstract: The present invention relates to a nucleic acid molecule encoding a polypeptide having a fluorescence emission activity with a maximum emission at 505 to 515 nm, wherein said nucleic acid molecule is selected from the group consisting of (a) a nucleic acid molecule encoding a polypeptide having the amino acid sequence of SEQ ID NO: 2; (b) a nucleic acid molecule having the DNA sequence of SEQ ID NO: 1; (c) a nucleic acid molecule hybridizing under stringent conditions to the complementary strand of (i.
    Type: Grant
    Filed: August 1, 2008
    Date of Patent: June 3, 2014
    Assignee: Helmholtz Zentrum Munchen Deutsches Forschungszentrum fur Gesundheit und Umwelt (GmbH)
    Inventors: Jean-Marie Buerstedde, Hiroshi Arakawa
  • Methods for Genetic Diversification in Gene Conversion Active Cells
    Publication number: 20140068795
    Abstract: The invention relates to a modified lymphoid cell having gene conversion fully or partially replaced by hypermutation, wherein said cell has no deleterious mutations in genes encoding paralogues and analogues of the RAD51 protein, and wherein said cell is capable of directed and selective genetic diversification of a target nucleic acid by hypermutation or a combination of hypermutation and gene conversion. The invention also relates to a method for diversifying any transgenic target gene in said cell. Preferably, the target gene is integrated into the immunoglobulin light or heavy chain locus by targeted integration.
    Type: Application
    Filed: August 2, 2013
    Publication date: March 6, 2014
    Inventors: Jean-Marie BUERSTEDDE, Hiroshi ARAKAWA
  • Methods for genetic diversification in gene conversion active cells
    Patent number: 8518699
    Abstract: The invention relates to a modified lymphoid cell having gene conversion fully or partially replaced by hypermutation, wherein said cell has no deleterious mutations in genes encoding paralogues and analogues of the RAD51 protein, and wherein said cell is capable of directed and selective genetic diversification of a target nucleic acid by hypermutation or a combination of hypermutation and gene conversion. The invention also relates to a method for diversifying any transgenic target gene in said cell. Preferably, the target gene is integrated into the immunoglobulin light or heavy chain locus by targeted integration.
    Type: Grant
    Filed: February 23, 2005
    Date of Patent: August 27, 2013
    Assignee: GSF-Forschungszentrum fur Umwelt und Gesundheit, GmbH
    Inventors: Jean-Marie Buerstedde, Hiroshi Arakawa
  • CIS-ACTING DIVERSIFICATION ACTIVATOR AND METHOD FOR SELECTIVE DIVERSIFICATION OF NUCLEIC ACIDS
    Publication number: 20110262902
    Abstract: The invention relates to identification of a cis-acting diversification activator (DIVAC) that is necessary and sufficient for the activation of diversification in transcription units linked thereto. The invention provides a method for diversification of a target nucleic acid comprising introducing a genetic construct comprising the diversification activator into a recipient cell, wherein the diversification activator is linked to the target nucleic acid.
    Type: Application
    Filed: April 9, 2009
    Publication date: October 27, 2011
    Applicant: Heimholz Zentrum Munchen Deutsches Forschungszen- trum fur Gesundheit und Umwelt (GmbH
    Inventors: Randolph B Caldwell, Jean-Marie Buerstedde, Ulrike Schötz
  • FLUORESCENT GFP VARIANT DISPLAYING HIGHLY INCREASED FLUORESCENCE INTENSITY WITHOUT A SPECTRAL SHIFT
    Publication number: 20110059443
    Abstract: The present invention relates to a nucleic acid molecule encoding a polypeptide having a fluorescence emission activity with a maximum emission at 505 to 515 nm, wherein said nucleic acid molecule is selected from the group consisting of (a) a nucleic acid molecule encoding a polypeptide having the amino acid sequence of SEQ ID NO: 2; (b) a nucleic acid molecule having the DNA sequence of SEQ ID NO: 1; (c) a nucleic acid molecule hybridizing under stringent conditions to the complementary strand of (i.
    Type: Application
    Filed: August 1, 2008
    Publication date: March 10, 2011
    Applicant: Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)
    Inventors: Jean-Marie Buerstedde, Hiroshi Arakawa
  • Methods for genetic diversification in gene conversion active cells
    Publication number: 20070186292
    Abstract: The invention relates to a modified lymphoid cell having gene conversion fully or partially replaced by hypermutation, wherein said cell has no deleterious mutations in genes encoding paralogues and analogues of the RAD51 protein, and wherein said cell is capable of directed and selective genetic diversification of a target nucleic acid by hypermutation or a combination of hypermutation and gene conversion. The invention also relates to a method for diversifying any transgenic target gene in said cell. Preferably, the target gene is integrated into the immunoglobulin light or heavy chain locus by targeted integration.
    Type: Application
    Filed: February 23, 2005
    Publication date: August 9, 2007
    Inventors: Jean-Marie Buerstedde, Hiroshi Arakawa