Abstract: Process for in vitro production of a culture or cell line infected by a viral strain associated with multiple sclerosis (MS), according to which a body sample is taken from an individual suffering from MS, the sample is cultivated in a culture medium which promotes the growth of infected cells to obtain a culture of primary infected cells, and a sample of the culture of primary cells or of a subculture of the latter is cultivated in series, that is to say by successive passages, in the culture medium to obtain the culture or cell line infected by a virus associated with MS. The process includes a procedure in which the culture medium also contains a beta anti-interferon antibody or an antibody which is directed against an antigenically close molecule, the antibody playing an inhibiting role in viral expression and allowing long-lasting expression and propagation of the viral strain in the culture or cell line.

Abstract: In a process for the in vitro production of a culture or cell line infected by a viral strain associated with multiple sclerosis (MS), a body sample is taken from an individual suffering from MS. The sample is cultivated in a culture medium that promotes the growth of infected cells to obtain a culture of primary infected cells. A sample of the culture of primary cells or a subculture of the latter is cultivated in series, by successive passages in the culture medium to obtain the culture or cell line infected by a virus associated with MS. The culture medium also contains a beta-interferon antibody or an antibody that is directed against an antigenically close molecule, the antibody playing an inhibiting role in viral expression and allowing long-lasting expression and propagation of the viral strain in the culture or cell line.

Abstract: The present invention relates to a process for in vitro culture of cells infected by a virus associated with multiple sclerosis and to the infected cell lines thus produced. According to the invention, the process includes: a) cultivation of human cells infected by a viral strain to obtain at least one culture of primary cells infected by the viral strain, b) cultivation of non-infected human cells permissive to the viral strain to obtain at least one permissive culture, c) cocultivation of at least one sample of a culture of infected primary cells and one sample of the permissive culture to obtain a first infected derived culture, d) cultivation in series of the first infected derived culture. The invention is used in particular in the pharmaceutical diagnostics industry sector.

Abstract: Process for in vitro production of a culture or cell line infected by a viral strain associated with multiple sclerosis (MS), according to which a body sample is taken from an individual suffering from MS, said sample is cultivated in a culture medium which promotes the growth of infected cells to obtain a culture of primary infected cells, and a sample of the culture of primary cells or of a subculture of the latter is cultivated in series, that is to say by successive passages, in said culture medium to obtain the culture or cell line infected by a virus associated with MS, which comprises a procedure in which the culture medium also contains a beta anti-interferon antibody or an antibody which is directed against an antigenically close molecule, the antibody playing an inhibiting role in viral expression and allowing long-lasting expression and propagation of the viral strain in the culture or cell line.

Abstract: The present invention relates to a process for in vitro culture of cells infected by a virus associated with multiple sclerosis and to the infected cell lines thus produced. The process is the cultivation of human cells infected by a viral strain to obtain at least one culture of primary cells infected by the said viral strain, along with the cultivation of non-infected human cells permissive to the viral strain to obtain at least one permissive culture, followed by cocultivation of at least one sample of a culture of infected primary cells and one sample of the permissive culture to obtain a first infected derived culture, then cultivating in series of the first infected derived culture. The invention is used in particular in the pharmaceutical diagnostics industry sector. In the preferred process, the infected cells are leptomeningeal cells and the permissive cells are leptomeningeal cells or plexuschoroideus cells.