Abstract: A device for non-destructive analysis of plants includes a unit transmitting a calibrated excitation laser beam, a unit measuring and imaging the fluorescence emitted by the irradiated plant(s) and a unit for digitally processing, storing and editing or displaying the collected images. The transmitting unit provides at least two different laser excitation wavelengths simultaneously and the measuring and imaging unit includes a unit for forming, from a common fluorescence beam emitted by the plant(s), simultaneously or successively, at least two secondary beams, each having its own wavelength and each constituting a fluorescence image at a matrix sensor, over the whole surface thereof or over part of the surface thereof, each image being separate for each secondary beam.