Abstract: A valve delivery device and method of use is provided. In one embodiment, a method for attaching a valve prosthesis to a target tissue includes providing a ring having a portion defining a groove and a plurality of fasteners coupled to the ring. A portion of the valve prosthesis is placed in the groove on the ring. The valve prosthesis portion in the groove is then secured to the ring. The ring is mounted on a delivery device and the delivery device is positioned so that the fasteners will engage the target tissue when the fasteners are deployed. A shaped plunger member may then deployed through an interior of the ring to swing said fasteners from a first position to a second tissue engagement position.

Abstract: The present invention provides a recombinant vector comprising, (i) a promoter sequence and (ii) a nucleotide sequence encoding a first protein which, when crystallized with a second protein, is capable of accommodating the second protein in the crystal lattice; said recombinant vector further allowing for the insertion of a further nucleotide sequence encoding a second protein to be located, when crystallized, in the crystal lattice of the first protein. The invention further provides a recombinant vector comprising (i) a promoter sequence and (ii) a nucleotide sequence encoding a first protein which upon crystallization yields crystals having available space in the lattice, so as to allow for the ordered packing of a second protein into the said available space, said recombinant vector further allowing, for the insertion of a further nucleotide sequence encoding a second protein to be accommodated, upon its crystallization, in the said available space in the lattice of the first protein.