Patents by Inventor Jeff M Byers

Jeff M Byers has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10761028
    Abstract: Methods and systems for determining extracellular concentration data of an analyte are disclosed. A method for determining extracellular concentration data of an analyte includes receiving sensor data from one or more arrays of functionalized plasmonic nanostructures on a localized surface plasmon resonance imaging chip in contact with a fluid containing at least one living cell for a plurality of times, determining intensity data for the one or more arrays, determining fractional occupancy based on the intensity data, and determining extracellular concentration data based on the fractional occupancy data. A system for determining extracellular concentration data of an analyte includes a LSPRi chip, a sensor component, an intensity component, a fractional occupancy component, a concentration component, and a processor to implement the components.
    Type: Grant
    Filed: June 20, 2016
    Date of Patent: September 1, 2020
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
  • Publication number: 20200158639
    Abstract: Methods and systems for determining extracellular concentration data of an analyte are disclosed. A method for determining extracellular concentration data of an analyte includes receiving sensor data from one or more arrays of functionalized plasmonic nanostructures on a localized surface plasmon resonance imaging chip in contact with a fluid containing at least one living cell for a plurality of times, determining intensity data for the one or more arrays, determining fractional occupancy based on the intensity data, and determining extracellular concentration data based on the fractional occupancy data. A system for determining extracellular concentration data of an analyte includes a LSPRi chip, a sensor component, an intensity component, a fractional occupancy component, a concentration component, and a processor to implement the components.
    Type: Application
    Filed: January 21, 2020
    Publication date: May 21, 2020
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
  • Patent number: 10641705
    Abstract: A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.
    Type: Grant
    Filed: October 16, 2017
    Date of Patent: May 5, 2020
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
  • Patent number: 10345287
    Abstract: A method for calibrating multiple nanostructures in parallel for quantitative biosensing using a chip for localized surface plasmon resonance (LSPR) biosensing and imaging. The chip is a glass coverslip compatible for use in a standard microscope with at least one array of functionalized plasmonic nanostructures patterned onto it using electron beam nanolithography. The chip is used to collect CCD-based LSPR imagery data of each individual nanostructure and LSPR spectral data of the array. The spectral data is used to determine the fractional occupancy of the array. The imagery data is modeled as a function of fractional occupancy to determine the fractional occupancy of each individual nanostructure.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: July 9, 2019
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers
  • Publication number: 20190162662
    Abstract: A method for measuring surface-induced cellular behavior that includes one or more lithographically patterned, functionalizable structures on a substrate, for example gold islands or grooved quartz, in contact with a fluid and in registry with at least one living cell for a plurality of times. The structures' shape, height, pitch and ordering are controlled by the lithographic process, such that the physical cues imparted to the cell by topography can be tuned independently of the chemical biofunctionality which is subsequently imparted via surface chemistry. Cellular behavior data, such as adhesion, migration, differentiation, division, secretion, apoptosis and necrosis, is measured using imaging sensors in relation to the surface topography and surface chemistry for a plurality of times.
    Type: Application
    Filed: November 20, 2018
    Publication date: May 30, 2019
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Marc Christophersen, Jeff M. Byers
  • Publication number: 20180164309
    Abstract: A chip for localized surface plasmon resonance (LSPR) biosensing and imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography. The nanostructures can be regenerated allowing the chip to be used multiple times. Also disclosed is a method for determining the fractional occupancy values for surface-bound receptors as a function of time for LSPR biosensing from the spectroscopic response of the array and modeling the photon count in each spectrometer channel, allowing for a functional relationship to be determined between the acquired spectrum and the fractional occupancy of binding sites on the array. Additionally disclosed is a method for the spatiotemporal mapping of receptor-ligand binding kinetics in LSPR imaging using the chip and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array.
    Type: Application
    Filed: January 29, 2018
    Publication date: June 14, 2018
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers
  • Patent number: 9915654
    Abstract: A chip for localized surface plasmon resonance (LSPR) biosensing and imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography. The nanostructures can be regenerated allowing the chip to be used multiple times. Also disclosed is a method for determining the fractional occupancy values for surface-bound receptors as a function of time for LSPR biosensing from the spectroscopic response of the array and modeling the photon count in each spectrometer channel, allowing for a functional relationship to be determined between the acquired spectrum and the fractional occupancy of binding sites on the array. Additionally disclosed is a method for the spatiotemporal mapping of receptor-ligand binding kinetics in LSPR imaging using the chip and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: March 13, 2018
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers
  • Publication number: 20180038791
    Abstract: A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.
    Type: Application
    Filed: October 16, 2017
    Publication date: February 8, 2018
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
  • Patent number: 9791368
    Abstract: A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: October 17, 2017
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
  • Publication number: 20160370290
    Abstract: Methods and systems for determining extracellular concentration data of an analyte are disclosed. A method for determining extracellular concentration data of an analyte includes receiving sensor data from one or more arrays of functionalized plasmonic nanostructures on a localized surface plasmon resonance imaging chip in contact with a fluid containing at least one living cell for a plurality of times, determining intensity data for the one or more arrays, determining fractional occupancy based on the intensity data, and determining extracellular concentration data based on the fractional occupancy data. A system for determining extracellular concentration data of an analyte includes a LSPRi chip, a sensor component, an intensity component, a fractional occupancy component, a concentration component, and a processor to implement the components.
    Type: Application
    Filed: June 20, 2016
    Publication date: December 22, 2016
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
  • Publication number: 20140273002
    Abstract: A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.
    Type: Application
    Filed: March 13, 2014
    Publication date: September 18, 2014
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
  • Publication number: 20140093977
    Abstract: A chip for localized surface plasmon resonance (LSPR) biosensing and imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography. The nanostructures can be regenerated allowing the chip to be used multiple times. Also disclosed is a method for determining the fractional occupancy values for surface-bound receptors as a function of time for LSPR biosensing from the spectroscopic response of the array and modeling the photon count in each spectrometer channel, allowing for a functional relationship to be determined between the acquired spectrum and the fractional occupancy of binding sites on the array. Additionally disclosed is a method for the spatiotemporal mapping of receptor-ligand binding kinetics in LSPR imaging using the chip and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array.
    Type: Application
    Filed: September 27, 2013
    Publication date: April 3, 2014
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers
  • Publication number: 20140095100
    Abstract: A method for calibrating multiple nanostructures in parallel for quantitative biosensing using a chip for localized surface plasmon resonance (LSPR) biosensing and imaging. The chip is a glass coverslip compatible for use in a standard microscope with at least one array of functionalized plasmonic nanostructures patterned onto it using electron beam nanolithography. The chip is used to collect CCD-based LSPR imagery data of each individual nanostructure and LSPR spectral data of the array. The spectral data is used to determine the fractional occupancy of the array. The imagery data is modeled as a function of fractional occupancy to determine the fractional occupancy of each individual nanostructure.
    Type: Application
    Filed: September 27, 2013
    Publication date: April 3, 2014
    Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers
  • Patent number: 8354929
    Abstract: A method and system for detecting and classifying intruders is provided. A noise threshold can be determined and set based on background noise. A seismic sensor can be configured to receive a plurality of seismic data signals. A microcontroller can be configured to count the number of times the noise threshold is exceeded over a defined time interval by the plurality of seismic data signals, and then detect and classify the presence of an intruder based on the count. Additionally, an amplitude evaluation module can be configured to determine a signal amplitude for the seismic data signals associated with the detected intruder and compare the detected intruder signal amplitude to known signal amplitudes in order to determine a sub-type of the intruder. Finally, a transmission source can be configured to transmit intruder detection and classification information to a remote location.
    Type: Grant
    Filed: September 9, 2009
    Date of Patent: January 15, 2013
    Assignee: The United States of America, as respresented by the Secretary of the Navy
    Inventors: Peter C Herdic, Robert M Baden, Brian H Houston, Phillip A Frank, Alain R Berdoz, Jeff M Byers
  • Publication number: 20100085188
    Abstract: A method and system for detecting and classifying intruders is provided. A noise threshold can be determined and set based on background noise. A seismic sensor can be configured to receive a plurality of seismic data signals. A microcontroller can be configured to count the number of times the noise threshold is exceeded over a defined time interval by the plurality of seismic data signals, and then detect and classify the presence of an intruder based on the count. Additionally, an amplitude evaluation module can be configured to determine a signal amplitude for the seismic data signals associated with the detected intruder and compare the detected intruder signal amplitude to known signal amplitudes in order to determine a sub-type of the intruder. Finally, a transmission source can be configured to transmit intruder detection and classification information to a remote location.
    Type: Application
    Filed: September 9, 2009
    Publication date: April 8, 2010
    Inventors: Peter C. Herdic, Robert M. Baden, Brian H. Houston, Phillip A. Frank, Alain R. Berdoz, Jeff M. Byers